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Epigenetics and Nuclear Signaling Histones HMGs

Anti-HMGB1 antibody [1F3] (ab190377)

Price and availability

268 032 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-HMGB1 antibody [1F3] (ab190377)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [1F3] to HMGB1
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-HMGB1 antibody [1F3]
    See all HMGB1 primary antibodies
  • Description

    Mouse monoclonal [1F3] to HMGB1
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length protein corresponding to Human HMGB1 aa 1 to the C-terminus. purified form E. coli.
    Database link: P09429

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HeLa cell crude extract; HeLa cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    1F3
  • Isotype

    IgG2b
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • HMGs
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HMG Box

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-HMGB1 antibody [1F3] (ab190377)
    Immunocytochemistry/ Immunofluorescence - Anti-HMGB1 antibody [1F3] (ab190377)

    Immunofluorescent analysis of HeLa cells labeling HMGB1 with ab190377 at 1/1000 dilution (red) and chicken polyclonal antibody to Vimentin (green) and DNA (blue).

  • Western blot - Anti-HMGB1 antibody [1F3] (ab190377)
    Western blot - Anti-HMGB1 antibody [1F3] (ab190377)
    Anti-HMGB1 antibody [1F3] (ab190377) at 1/1000 dilution + HeLa cell crude extract

    Predicted band size: 25 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-HMGB1 antibody [1F3] (ab190377)
    Immunocytochemistry/ Immunofluorescence - Anti-HMGB1 antibody [1F3] (ab190377)

    Immunofluorescent analysis of HeLa cells staining HMGB1 using ab190377 at 1/2000 dilution (red) and rabbit polyclonal to GAPDH. 

  • Western blot - Anti-HMGB1 antibody [1F3] (ab190377)
    Western blot - Anti-HMGB1 antibody [1F3] (ab190377)
    All lanes : Anti-HMGB1 antibody [1F3] (ab190377) at 1/2000 dilution

    Lane 1 : NIH-3T3 cell lysates
    Lane 2 : C6 cell lysates
    Lane 3 : HEK293 cell lysates
    Lane 4 : HeLa cell lysates
    Lane 5 : SH-SY5Y cell lysates

    Predicted band size: 25 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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