Anti-HMGB1 antibody [1F3] (ab190377)
Key features and details
- Mouse monoclonal [1F3] to HMGB1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2b
Overview
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Product name
Anti-HMGB1 antibody [1F3]
See all HMGB1 primary antibodies -
Description
Mouse monoclonal [1F3] to HMGB1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB MouseHuman -
Immunogen
Recombinant full length protein corresponding to Human HMGB1 aa 1 to the C-terminus. purified form E. coli.
Database link: P09429 -
Positive control
- HeLa cell crude extract; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
1F3 -
Isotype
IgG2b -
Research areas
Images
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Immunofluorescent analysis of HeLa cells labeling HMGB1 with ab190377 at 1/1000 dilution (red) and chicken polyclonal antibody to Vimentin (green) and DNA (blue).
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Anti-HMGB1 antibody [1F3] (ab190377) at 1/1000 dilution + HeLa cell crude extract
Predicted band size: 25 kDa
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Immunofluorescent analysis of HeLa cells staining HMGB1 using ab190377 at 1/2000 dilution (red) and rabbit polyclonal to GAPDH.
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All lanes : Anti-HMGB1 antibody [1F3] (ab190377) at 1/2000 dilution
Lane 1 : NIH-3T3 cell lysates
Lane 2 : C6 cell lysates
Lane 3 : HEK293 cell lysates
Lane 4 : HeLa cell lysates
Lane 5 : SH-SY5Y cell lysates
Predicted band size: 25 kDa