Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HLTF antibody [EPR14761] (ab183042)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14761] to HLTF
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-HLTF antibody [EPR14761]
    See all HLTF primary antibodies

  • Description

    Rabbit monoclonal [EPR14761] to HLTF

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, K562 and Jurkat cell lysates; Human infiltrating duct carcinoma of breast and Human ovarian carcinoma tissues; Jurkat cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-HLTF antibody [EPR14761] (ab183042)
    Western blot - Anti-HLTF antibody [EPR14761] (ab183042)
    All lanes : Anti-HLTF antibody [EPR14761] (ab183042) at 1 µg

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : HLTF knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 114 kDa
    Observed band size: 113 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 3: Merged signal (red and green). Green - ab183042 observed at 113 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab183042 was shown to specifically react with HLTF in wild-type HAP1 cells as signal was lost in HLTF knockout cells. Wild-type and HLTF knockout samples were subjected to SDS-PAGE. Ab183042 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-HLTF antibody [EPR14761] (ab183042)
    Immunocytochemistry/ Immunofluorescence - Anti-HLTF antibody [EPR14761] (ab183042)

    Immunofluorescent analysis of Jurkat cells (-20°C Acetone-fixed) labeling HLTF with ab183042 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Western blot - Anti-HLTF antibody [EPR14761] (ab183042)
    Western blot - Anti-HLTF antibody [EPR14761] (ab183042)
    All lanes : Anti-HLTF antibody [EPR14761] (ab183042) at 1/50000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : K562 lysate
    Lane 3 : Jurkat lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 114 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLTF antibody [EPR14761] (ab183042)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLTF antibody [EPR14761] (ab183042)

    Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling HLTF with ab183042 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLTF antibody [EPR14761] (ab183042)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLTF antibody [EPR14761] (ab183042)

    Immunohistochemical analysis of paraffin-embedded Human infiltrating duct carcinoma of breast tissue labeling HLTF with ab183042 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-HLTF antibody [EPR14761] (ab183042)
    Anti-HLTF antibody [EPR14761] (ab183042)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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