Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 02, 2021

Anti-HLTF antibody [EPR14761] (ab183042)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14761] to HLTF
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-HLTF antibody [EPR14761]
See all HLTF primary antibodies
Description

Rabbit monoclonal [EPR14761] to HLTF
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- HeLa, K562 and Jurkat cell lysates; Human infiltrating duct carcinoma of breast and Human ovarian carcinoma tissues; Jurkat cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14761
Isotype

IgG
Research areas

Western blot - Anti-HLTF antibody [EPR14761] (ab183042)

All lanes : Anti-HLTF antibody [EPR14761] (ab183042) at 1 µg

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : HLTF knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 114 kDa
Observed band size: 113 kDa
why is the actual band size different from the predicted?

Lanes 1 - 3: Merged signal (red and green). Green - ab183042 observed at 113 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab183042 was shown to specifically react with HLTF in wild-type HAP1 cells as signal was lost in HLTF knockout cells. Wild-type and HLTF knockout samples were subjected to SDS-PAGE. Ab183042 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-HLTF antibody [EPR14761] (ab183042)

Immunofluorescent analysis of Jurkat cells (-20°C Acetone-fixed) labeling HLTF with ab183042 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Western blot - Anti-HLTF antibody [EPR14761] (ab183042)

All lanes : Anti-HLTF antibody [EPR14761] (ab183042) at 1/50000 dilution

Lane 1 : HeLa lysate
Lane 2 : K562 lysate
Lane 3 : Jurkat lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 114 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLTF antibody [EPR14761] (ab183042)

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling HLTF with ab183042 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLTF antibody [EPR14761] (ab183042)

Immunohistochemical analysis of paraffin-embedded Human infiltrating duct carcinoma of breast tissue labeling HLTF with ab183042 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Anti-HLTF antibody [EPR14761] (ab183042)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com