Anti-Proteasome 20S alpha + beta antibody (ab22673)
Key features and details
- Rabbit polyclonal to Proteasome 20S alpha + beta
- Suitable for: ICC/IF
- Reacts with: Human, Saccharomyces cerevisiae
- Isotype: IgG
Overview
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Product name
Anti-Proteasome 20S alpha + beta antibody -
Description
Rabbit polyclonal to Proteasome 20S alpha + beta -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human, Saccharomyces cerevisiae -
Immunogen
Tissue/ cell preparation: Proteasomal preparation isolated from human red blood cells.
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General notes
Dilute to working concentration in PBS pH 7.2- 7.4, and store at 2 to 4°C (do not freeze). Use diluted antibody within 1 month.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.065% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha + beta antibody (ab22673) This image is courtesy of an anonymous abreview.
ab22673 staining human Hela cells by ICC/IF. Cells were formaldehyde fixed, permeabilized in 0.1% Triton and blocked in 3% BSA for 15 minutes at room temperature. The primary antibody was diluted 1/2000 (3% BSA) and incubated with sample for 1 hour. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit, diluted 1/1000 was used as secondary.
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Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha + beta antibody (ab22673) Image from Anderson DD et al., J Biol Chem. 2012 Feb 10;287(7):4790-9. Epub 2011 Dec 21. Fig 5.; doi: 10.1074/jbc.M111.302174; February 10, 2012 The Journal of Biological Chemistry, 287, 4790-4799.Immunofluorescence analysis of HeLa cells, staining Proteasome 20S alpha + beta with ab22673.
Cells were fixed with formaldehyde and permeabilized using 0.1% Triton X-100. Samples were incubated with primary antibody (1/1000) fr 1 hour at room temperature. An AlexaFluor®555-conjugated donkey anti-rabbit IgG was used as the secondary antibody.