Anti-HIV1 p24 antibody [39/6.14] (ab9072)
![Anti-HIV1 p24 antibody [39/6.14] (ab9072)](https://www.abcam.com/ps/products/9/ab9072/Images/ab9072-16747-anti-hiv1-p24-antibody-39-614-western-blot.jpg "Anti-HIV1 p24 antibody [39/6.14] (ab9072)")
Key features and details
- Mouse monoclonal [39/6.14] to HIV1 p24
- Suitable for: ICC/IF, WB, Radioimmunoprecipitation, ELISA, Sandwich ELISA
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-HIV1 p24 antibody [39/6.14]
See all HIV1 p24 primary antibodies -
Description
Mouse monoclonal [39/6.14] to HIV1 p24 -
Host species
Mouse -
Specificity
Reacts with Human Immunodeficiency Virus Type 1(HIV 1) p24 protein. Cross reacts with Human Immunodeficiency Virus Type 2 and Simian Immunodeficiency Virus (SIV). -
Tested applications
Suitable for: ICC/IF, WB, Radioimmunoprecipitation, ELISA, Sandwich ELISAmore details -
Species reactivity
Reacts with: Species independent -
General notes
The antibody is used to detect HIV 1 core protein in virus infected cells as well as in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis. This antibody is useful as solid phase for capture of p24 in an antigen ELISA format
Properties
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Form
Liquid -
Storage instructions
Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from serum-free culture supernatant. -
Primary antibody notes
The antibody is used to detect HIV 1 core protein in virus infected cells as well as in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis. This antibody is useful as solid phase for capture of p24 in an antigen ELISA format -
Clonality
Monoclonal -
Clone number
39/6.14 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-HIV1 p24 antibody [39/6.14] (ab9072)All lanes : Anti-HIV1 p24 antibody [39/6.14] (ab9072) at 1/1000 dilution
Lane 1 : Untransfected 293T cells
Lane 2 : Human 293T cells transfected with HIV proviral DNA
Secondary
All lanes : Goat anti-mouse HRP conjugated antibody
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 24,45 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesAb9072 in Western blot.
This image is courtesy of an anonymous Abreview submitted on 13 February 2006.
