Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (ab275929)
![Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (ab275929)](https://www.abcam.com/ps/products/275/ab275929/Images/ab275929-411477-antihistoneh3antibodywesternblot.png "Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (ab275929)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM157] to Histone H3.3 (tri methyl K79)
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
Overview
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Product name
Anti-Histone H3.3 (tri methyl K79) antibody [RM157]
See all Histone H3.3 primary antibodies -
Description
Rabbit monoclonal [RM157] to Histone H3.3 (tri methyl K79) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Recombinant fragment
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Immunogen
Synthetic peptide corresponding to Human Histone H3.3 (tri methyl K79).
Database link: P84243 -
Positive control
- WB: Acid extracts of HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA, 48.01% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
RM157 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (ab275929)All lanes : Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (ab275929) at 0.5 µg/ml
Lane 1 : Recombinant histone H3.3
Lane 2 : Acid extracts of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells
Predicted band size: 15 kDa
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![Dot Blot - Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (ab275929)](https://www.abcam.com/ps/products/275/ab275929/Images/ab275929-411479-antihistoneh3antibodypeptideblot.png)
Dot Blot - Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (ab275929)A Peptide dotblot shows ab275929 only reacts to Histone H3 trimethyl-Lysine 79 (K79me3). No cross reactivity. with non-modified Lysine 79 (H3K79Ctrl), monomethylated. Lysine 79 (K79me1), or dimethylated Lysine 79 (K79me2).
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![Peptide Array - Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (ab275929)](https://www.abcam.com/ps/products/275/ab275929/Images/ab275929-411478-antihistoneh3antibodypeptidearray.png)
Peptide Array - Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (ab275929)ab275929 specifically reacts to Histone H3 trimethylated at Lysine 79 (K79me3). No cross reactivity with other methylated lysines in Histone H3.
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![Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (ab275929)](https://www.abcam.com/ps/products/275/ab275929/Images/ab275929-4-benefits-of-recombinant-antibodies.png)
Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (ab275929)
