Anti-Histone H3 (unmodified K4) antibody [RM186] - N-terminal (ab238310)
![Anti-Histone H3 (unmodified K4) antibody [RM186] - N-terminal (ab238310)](https://www.abcam.com/ps/products/238/ab238310/Images/ab238310-316143-anti-histone-h3-unmodified-k4-antibody-rm186-n-terminal-western-blot.png "Anti-Histone H3 (unmodified K4) antibody [RM186] - N-terminal (ab238310)")
Key features and details
- Rabbit monoclonal [RM186] to Histone H3 (unmodified K4) - N-terminal
- Suitable for: ELISA, WB, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (unmodified K4) antibody [RM186] - N-terminal
See all Histone H3 primary antibodies -
Description
Rabbit monoclonal [RM186] to Histone H3 (unmodified K4) - N-terminal -
Host species
Rabbit -
Specificity
ab238310 recognizes Histone H3 unmodified at Lys4 and does not recognize acetylated or methylated Lys4. The antibody binding specificity allows for modifications of Arg2, Thr3, and/or other modifications in Histone H3. -
Tested applications
Suitable for: ELISA, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Synthetic peptide corresponding to Human Histone H3 (N terminal) (unmodified K4).
Database link: Q16695 -
Positive control
- WB: Acid extracts of HeLa cells. ICC/IF: HeLa cells treated with sodium butyrate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM186 -
Isotype
IgG
Images
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Western blot - Anti-Histone H3 (unmodified K4) antibody [RM186] - N-terminal (ab238310)All lanes : Anti-Histone H3 (unmodified K4) antibody [RM186] - N-terminal (ab238310) at 1 µg/ml
Lane 1 : Acid extracts of HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with sodium butyrate
Lane 2 : Acid extracts of HeLa cells treated with Nocodazole
Lane 3 : Acid extracts of HeLa cells
Lane 4 : Recombinant Histone H3.1
Predicted band size: 15 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (unmodified K4) antibody [RM186] - N-terminal (ab238310)](https://www.abcam.com/ps/products/238/ab238310/Images/ab238310-316144-anti-histone-h3-unmodified-k4-antibody-rm186-n-terminal-immunofluorescence.png)
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (unmodified K4) antibody [RM186] - N-terminal (ab238310)HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with sodium butyrate stained for Histone H3 (unmodified K4) (red) using ab238310 at 2 µg/ml in ICC/IF. Actin filaments have been labeled with fluorescein phalloidin (green).
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![ELISA - Anti-Histone H3 (unmodified K4) antibody [RM186] - N-terminal (ab238310)](https://www.abcam.com/ps/products/238/ab238310/Images/ab238310-316142-anti-histone-h3-unmodified-k4-antibody-rm186-n-terminal-elisa.png)
ELISA - Anti-Histone H3 (unmodified K4) antibody [RM186] - N-terminal (ab238310)ab238310 specifically recognizes Histone H3 unmodified at Lys4 and does not recognize acetylated, mono-methylated, dimethylated, or trimethylated Lys4. The antibody binding specificity allows for modifications of Arg2 or Thr3 in histone H3.
