Anti-Histone H3 (tri methyl K27) antibody - ChIP Grade (ab195477)
Key features and details
- Rabbit polyclonal to Histone H3 (tri methyl K27) - ChIP Grade
- Suitable for: WB, ICC/IF, Dot blot, ChIP, ChIP-sequencing
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (tri methyl K27) antibody - ChIP Grade
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (tri methyl K27) - ChIP Grade -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Dot blot, ChIP, ChIP-sequencingmore details -
Species reactivity
Reacts with: Mouse, Human, Recombinant fragment
Predicted to work with: Rat, Arabidopsis thaliana, Drosophila melanogaster, Zebrafish, Schistosoma japonicum
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Immunogen
Synthetic peptide corresponding to Human Histone H3 (tri methyl K27) conjugated to keyhole limpet haemocyanin.
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Positive control
- Chromatin prepared from HeLa cells; Chromatin prepared from HeLaS3 cells; HeLa whole cell extract; HeLa histone extract; NIH 3T3 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.05% Sodium azide, 0.05% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ChIP-sequencing - Anti-Histone H3 (tri methyl K27) antibody - ChIP Grade (ab195477)
ChIPseq results obtained with ab195477 directed against Histone H3 (tri methyl K27).
ChIP was performed on sheared chromatin from 1 million HeLaS3 cells using 1 µg of ab195477. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure shows the enrichment in genomic regions of chromosome 6, surrounding the TSH2B gene (indicated by an arrow; fig A), of chromosome 20, surrounding the MYT1 gene (fig B), and of chromosome 2 and 3 (figure C and D).
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ChIP - Anti-Histone H3 (tri methyl K27) antibody - ChIP Grade (ab195477)ChIP results obtained with ab195477 directed against Histone H3 (tri methyl K27).
ChIP assays were performed using human HeLa cells, ab195477 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the active genes EIF4A2 and GAPDH as negative controls, and for the coding regions of the inactive genes MYT1 and TSH2B as positive controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K27me3 is preferably present at inactive genes.
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K27) antibody - ChIP Grade (ab195477)Immunofluorescent analysis of NIH 3T3 cells labeling Histone H3 (tri methyl K27) with ab195477 at 1/200 dilution. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with ab195477 in blocking solution followed by an ant-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
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Dot Blot - Anti-Histone H3 (tri methyl K27) antibody - ChIP Grade (ab195477)Dot Blot analysis was performed to test the cross reactivity of ab195477 against Histone H3 (tri methyl K27) with peptides containing other modifications of Histone H3 and H4 and the unmodified H3K27 sequence. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1/5000. Figure shows a high specificity of the antibody for the modification of interest. Please note that that antibody also recognizes the modification if S28 is phosphorylated.
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Western blot - Anti-Histone H3 (tri methyl K27) antibody - ChIP Grade (ab195477)All lanes : Anti-Histone H3 (tri methyl K27) antibody - ChIP Grade (ab195477) at 1/500 dilution
Lane 1 : HeLa whole cell extract at 25 µg
Lane 2 : HeLa histone extract at 15 µg
Lane 3 : Recombinant Histone H2A at 1 µg
Lane 4 : Recombinant Histone H2B at 1 µg
Lane 5 : Recombinant Histone H3 at 1 µg
Lane 6 : Recombinant Histone H4 at 1 µg
Predicted band size: 15 kDaThe antibody was diluted in TBS-Tween containing 5% skimmed milk.
