Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Histone H3 (phospho T3) antibody [RM159] (ab222775)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [RM159] to Histone H3 (phospho T3)
Suitable for: ICC/IF, WB
Reacts with: Human, Recombinant fragment

Product name

Anti-Histone H3 (phospho T3) antibody [RM159]
See all Histone H3 primary antibodies
Description

Rabbit monoclonal [RM159] to Histone H3 (phospho T3)
Host species

Rabbit
Specificity

ab222775 reacts to Histone H3 phosphorylated at Threonine 3. No cross reactivity with other phosphorylated histones
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to Histone H3 (phospho T3).
Positive control
- WB: HeLa cell lysate, non-treated or treated with Nocodazole. ICC/IF: HeLa cells.

Application	Species
ICC/IF	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Purification notes

ab222775 was purified from an animal origin–free culture supernatant.
Clonality

Monoclonal
Clone number

RM159
Isotype

IgG
Research areas

Western blot - Anti-Histone H3 (phospho T3) antibody [RM159] (ab222775)

All lanes : Anti-Histone H3 (phospho T3) antibody [RM159] (ab222775) at 0.1 µg/ml

Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate, non-treated
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with Nocodazole
Lane 3 : Recombinant Histone H3.3

Predicted band size: 15 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho T3) antibody [RM159] (ab222775)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H3 (red) using ab222775 at 2 µg/ml in ICC/IF.

Actin filaments were labeled with fluorescein phalloidin (green).
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho T3) antibody [RM159] (ab222775)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H3 (red) using ab222775 at 2 µg/ml in ICC/IF.

Actin filaments were labeled with fluorescein phalloidin (green).
Anti-Histone H3 (phospho T3) antibody [RM159] (ab222775)

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