Anti-Histone H3 (phospho T3, acetyl K4) antibody (ab272140)
Key features and details
- Rabbit polyclonal to Histone H3 (phospho T3, acetyl K4)
- Suitable for: Dot blot, ICC/IF, WB
- Reacts with: Mouse, Human, Caenorhabditis elegans
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (phospho T3, acetyl K4) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (phospho T3, acetyl K4) -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human, Caenorhabditis elegans -
Immunogen
Synthetic peptide corresponding to Human Histone H3 (propionyl T3, acetyl K4).
Database link: Q71DI3 -
Positive control
- ICC: HeLa cells; WB: HeLa and NIH/3T3 histone preparation. Caenorhabditis elegans embryo lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 antibody (ab272140)
Immunofluorescence analysis of HeLa cells labeling Histone H3 (green) using ab272140 at 1:100, followed by a Dylight 488 secondary antibody at 1:10,000. Localization: Histone H3 [ac Lys4, p Thr3] is nuclear and chromosomal. Countersatin: nuclei and alpha-tubulin were coexpressed with DAPI (blue) and Dylight 550 (red). Fixation: 0.5% PFA.
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Western blot - Anti-Histone H3 antibody (ab272140)All lanes : Anti-Histone H3 (phospho T3, acetyl K4) antibody (ab272140) at 1/500 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) histone preparation
Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) histone preparation
Lane 3 : Caenorhabditis elegans embryo lysate.
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : IRDye800™ rabbit secondary antibody at 1/10000 dilution
Predicted band size: 15 kDaBlock: 5% BLOTTO
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Dot Blot - Anti-Histone H3 antibody (ab272140)Dot Blot analysis of Histone H3 methylated forms using ab272140 at 1:1000, followed by a Dylight 488 secondary antibody at 1:10,000. Block: 5% BLOTTO.
Lane 1: pT3.
Lane 2: pT3/K4me1.
Lane 3: pT3/K4me2.
Lane 4: pT3/K4ac.
Lane 5: pT3/RZm2s.
Lane 6: K4 unmodified.
Lane 7: K4me1.
Lane 8: K4me2.
Lane 9: K4me3.
Lane 10: K4me ac.
Load: 1, 10, and 100 picomoles of peptide.