Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Histone H3 (phospho S10 + T11) antibody [E173] - BSA and Azide free (ab239803)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [E173] to Histone H3 (phospho S10 + T11) - BSA and Azide free
Suitable for: ELISA, Dot blot, WB, ICC/IF, IHC-P, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-Histone H3 (phospho S10 + T11) antibody [E173] - BSA and Azide free
See all Histone H3 primary antibodies
Description

Rabbit monoclonal [E173] to Histone H3 (phospho S10 + T11) - BSA and Azide free
Host species

Rabbit

Tested Applications & Species

Application	Species
Dot	Recombinant fragment
ELISA	Recombinant fragment
ICC/IF	Human
IHC-P	Human
IP	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IP: HeLa cell lysate
General notes
Ab239803 is the carrier-free version of ab32107. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab239803 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

E173
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S10 + T11) antibody [E173] - BSA and Azide free (ab239803)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling Histone H3 (phospho S10 + T11) with ab32107 at a dilution of 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000, 2 μg/mL) was used as the secondary antibody. Cells were counter-stained with ab195889 Anti-Alpha Tubulin antibody [DM1A] (1/200, 2.5 μg/mL) - Microtubule Marker (Alexa Fluor^® 594). DAPI (blue) was used as a nuclear counterstain.
Confocal image showing nuclear staining on mitotic HeLa cell.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32107).
ELISA - Anti-Histone H3 (phospho S10 + T11) antibody [E173] - BSA and Azide free (ab239803)

Direct ELISA antibody dose-response curve using ab32107 (0-1000 ng/mL).

Peptides - Histone H3 unmodified peptide, Histone H3 phospho S10 peptide, Histone H3 phospho T11 peptide (10 ng/mL).

Secondary antibody - Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (1/2500).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32107).
Immunoprecipitation - Anti-Histone H3 (phospho S10 + T11) antibody [E173] - BSA and Azide free (ab239803)

Purified ab32107 at 1/50 dilution (2µg) immunoprecipitating Histone H3 in HeLa treated with calyculin A whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) treated with calyculin A whole cell lysate 10µg
Lane 2 (+): ab32107 + HeLa treated with calyculin A whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32107 in HeLa treated with calyculin A whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 17 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32107).
ELISA - Anti-Histone H3 (phospho S10 + T11) antibody [E173] - BSA and Azide free (ab239803)

Direct ELISA antibody dose-response curve using ab32107 (0-1000 ng/mL).

Peptides - Histone H3 unmodified peptide, Histone H3 phospho S10 peptide, Histone H3 phospho T11 peptide (100 ng/mL).

Secondary antibody - Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (1/2500).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32107).
Dot Blot - Anti-Histone H3 (phospho S10 + T11) antibody [E173] - BSA and Azide free (ab239803)

Dot blot analysis of Histone H3 phospho S10 peptide (Lane 1), Histone H3 phospho T11 peptide (Lane 2) and Histone H3 unmodified peptide (Lane 3) labelling Histone H3 (phospho S10 + T11) with ab32107 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) was used as the secondary antibody at a dilution of 1/10 000.

Blocking and diluting buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32107).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho S10 + T11) antibody [E173] - BSA and Azide free (ab239803)

Ab32107, at a 1/50 dilution, staining Histone H3 (phospho S10 + T11) in paraffin embedded human lymphoma tissue by Immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32107).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S10 + T11) antibody [E173] - BSA and Azide free (ab239803) This image is courtesy of an Abreview submitted by Kirk Mcmanus.

ab32107 (1/1000) staining Histone H3 (phospho S10 + T11) in paraformaldehyde-fixed, DAPI counterstained HeLa cells. Secondary antibody: Goat anti-Rabbit IgG conjugated to Cy3® (1/200). Please refer to abreview for further details.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32107).
Anti-Histone H3 (phospho S10 + T11) antibody [E173] - BSA and Azide free (ab239803)

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