Anti-Histone H3 (mono methyl K27) antibody - ChIP Grade (ab194688)
Key features and details
- Rabbit polyclonal to Histone H3 (mono methyl K27) - ChIP Grade
- Suitable for: Dot blot, ChIP, IHC-P, WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (mono methyl K27) antibody - ChIP Grade
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (mono methyl K27) - ChIP Grade -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, ChIP, IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: a wide range of other species
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Immunogen
Synthetic peptide corresponding to Human Histone H3 (mono methyl K27).
Database link: Q16695 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K27) antibody (ab194688)
Paraffin-embedded human kidney cancer tissue stained for Histone H3 (mono methyl K27) using ab194688 at 1/200 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K27) antibody (ab194688)Immunofluorescence staining of 293T cells stained for Histone H3 (mono methyl K27) with ab194688. Nuclei are labeled with DAPI (Blue).
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Western blot - Anti-Histone H3 (mono methyl K27) antibody (ab194688)All lanes : Anti-Histone H3 (mono methyl K27) antibody - ChIP Grade (ab194688)
Lane 1 : HeLa cell lysate
Lane 2 : H3 protein
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Predicted band size: 16 kDaBlocking buffer: 3% nonfat dry milk in TBST. -
Dot Blot - Anti-Histone H3 (mono methyl K27) antibody (ab194688)Dot-blot analysis of methylation peptides using ab194688.
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ChIP - Anti-Histone H3 (mono methyl K27) antibody (ab194688)Chromatin immunoprecipitation analysis of extracts of 293T cells, using ab194688 and rabbit IgG. P1 and P2 were located on ANO2 gene. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mono methyl K27) antibody (ab194688)Paraffin-embedded rat brain tissue stained for Histone H3 (mono methyl K27) using ab194688 at 1/200 dilution in immunohistochemical analysis.
