Anti-Histone H3 (butyryl K23) antibody (ab241465)
Key features and details
- Rabbit polyclonal to Histone H3 (butyryl K23)
- Suitable for: WB, IP, ICC/IF, ICC, ChIP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (butyryl K23) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (butyryl K23) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IF, ICC, ChIPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Histone H3 (butyryl K23).
Database link: P68431 -
Positive control
- WB: HeLa, HEK-293, A549 and K562 whole cell lysates, treated with 30 mM sodium butyrate for 4 hours. ICC/IF: HeLa cells (treated with 30mM sodium butyrate for 4 hours). ICC: HeLa cells (treated with 30mM sodium butyrate for 4 hours). IP: HeLa whole cell lysate. ChIP: Chromatin prepared from HeLa cells (treated with 30mM sodium butyrate for 4 hours).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Images
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Western blot - Anti-Histone H3 (butyryl K23) antibody (ab241465)All lanes : Anti-Histone H3 (butyryl K23) antibody (ab241465) at 1/2000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 4 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 5 : HeLa whole cell lysate, untreated
Lane 6 : HEK-293 whole cell lysate, untreated
Lane 7 : A549 whole cell lysate, untreated
Lane 8 : K562 whole cell lysate, untreated
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 15 kDa
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Immunocytochemistry - Anti-Histone H3 (butyryl K23) antibody (ab241465)HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 30 mM sodium butyrate for 4 hours) labeling Histone H3 (butyryl K23) with ab241465 at 1/20 dilution in ICC.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (butyryl K23) antibody (ab241465)HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 30 mM sodium butyrate for 4 hours) labeling Histone H3 (butyryl K23) with ab241465 at 1/10 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor®488-conjugated Goat anti-Rabbit IgG(H+L).
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Immunoprecipitation - Anti-Histone H3 (butyryl K23) antibody (ab241465)Histone H3 (butyryl K23) was immunoprecipitated from 500 µg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab241465 at 1/200 dilution.
All lanes : Anti-Histone H3 (butyryl K23) antibody (ab241465) at 1/1000 dilution
Lane 1 : Rabbit control IgG instead of ab241465 in HeLa whole cell lysate
Lane 2 : ab241465 IP in HeLa whole cell lysate
Lane 3 : HeLa whole cell lysate at 20 µg
Secondary
All lanes : HRP-conjugated Protein G at 1/2000 dilution
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ChIP - Anti-Histone H3 (butyryl K23) antibody (ab241465)HeLa (human epithelial cell line from cervix adenocarcinoma; (106, treated with 30mM sodium butyrate for 4 hours) cells were treated with micrococcal nuclease, sonicated, and immunoprecipitated with 5 μg ab241465 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
