Anti-Histone H2B (beta-hydroxybutyryl K20) antibody (ab242276)
Key features and details
- Rabbit polyclonal to Histone H2B (beta-hydroxybutyryl K20)
- Suitable for: WB, ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H2B (beta-hydroxybutyryl K20) antibody
See all Histone H2B primary antibodies -
Description
Rabbit polyclonal to Histone H2B (beta-hydroxybutyryl K20) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human Histone H2B (beta-hydroxybutyryl K20).
Database link: P62807 -
Positive control
- WB: HEK-293 and A549 whole cell lysates, treated with 50mM Sodium 3-hydroxybutyrate for 72 hours. ICC: HeLa cells (treated with 50mM sodium 3-hydroxybutyrate for 72 hours).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Images
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Western blot - Anti-Histone H2B (beta-hydroxybutyryl K20) antibody (ab242276)All lanes : Anti-Histone H2B (beta-hydroxybutyryl K20) antibody (ab242276) at 1/100 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate, treated (+) with 50mM Sodium 3-hydroxybutyrate for 72 hours
Lane 2 : A549 (human lung carcinoma cell line) whole cell lysate, treated (+) with 50mM Sodium 3-hydroxybutyrate for 72 hours
Lane 3 : HEK-293 whole cell lysate, untreated (-)
Lane 4 : A549 whole cell lysate, untreated (-)
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14 kDa
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Immunocytochemistry - Anti-Histone H2B (beta-hydroxybutyryl K20) antibody (ab242276)HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 50mM sodium 3-hydroxybutyrate for 72 hours) labeling Histone H2B (beta-hydroxybutyryl K20) with ab242276 at 1/40 dilution in ICC.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
