Anti-Histone H2B (acetyl K20) antibody [RM235] (ab271344)
![Anti-Histone H2B (acetyl K20) antibody [RM235] (ab271344)](https://www.abcam.com/ps/products/271/ab271344/Images/ab271344-384438-rabbit-monoclonal-antibody-rm235-wb-hela-cells.jpg "Anti-Histone H2B (acetyl K20) antibody [RM235] (ab271344)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM235] to Histone H2B (acetyl K20)
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-Histone H2B (acetyl K20) antibody [RM235]
See all Histone H2B primary antibodies -
Description
Rabbit monoclonal [RM235] to Histone H2B (acetyl K20) -
Host species
Rabbit -
Specificity
No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.
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Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Histone H2B (acetyl K20).
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Positive control
- ICC/IF: HeLa cells treated with sodium butyrate. WB: HeLa cells treated with sodium butyrate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
RM235 -
Isotype
IgG
Images
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Western blot - Anti-Histone H2B (acetyl K20) antibody [RM235] (ab271344)All lanes : Anti-Histone H2B (acetyl K20) antibody [RM235] (ab271344) at 0.5 µg/ml
Lane 1 : Acid extracts of HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with sodium butyrate
Lane 2 : Acid extracts of untreated HeLa cells
Predicted band size: 14 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K20) antibody [RM235] (ab271344)](https://www.abcam.com/ps/products/271/ab271344/Images/ab271344-384437-rabbit-monoclonal-antibody-rm235-icc-hela-cells.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K20) antibody [RM235] (ab271344)Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells treated with sodium butyrate stained for Histone H2B (acetyl K20) using ab271344 at 2 µg/ml (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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![ELISA - Anti-Histone H2B (acetyl K20) antibody [RM235] (ab271344)](https://www.abcam.com/ps/products/271/ab271344/Images/ab271344-384436-rabbitmonoclonalantibodyrm235elisa.jpg)
ELISA - Anti-Histone H2B (acetyl K20) antibody [RM235] (ab271344)ab271344 specifically reacts to Histone H2B acetylated at Lysine 20 (K20ac). No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.
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![Anti-Histone H2B (acetyl K20) antibody [RM235] (ab271344)](https://www.abcam.com/ps/products/271/ab271344/Images/ab271344-4-benefits-of-recombinant-antibodies.png)
Anti-Histone H2B (acetyl K20) antibody [RM235] (ab271344)