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Anti-Histone H2B (acetyl K20) antibody [RM235] (ab271344)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [RM235] to Histone H2B (acetyl K20)
  • Suitable for: WB, ELISA, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Histone H2B (acetyl K20) antibody [RM235]
    See all Histone H2B primary antibodies

  • Description

    Rabbit monoclonal [RM235] to Histone H2B (acetyl K20)

  • Host species

    Rabbit

  • Specificity

    No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Histone H2B (acetyl K20).

  • Positive control

    • ICC/IF: HeLa cells treated with sodium butyrate. WB: HeLa cells treated with sodium butyrate.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    RM235

  • Isotype

    IgG

Images

  • Western blot - Anti-Histone H2B (acetyl K20) antibody [RM235] (ab271344)
    Western blot - Anti-Histone H2B (acetyl K20) antibody [RM235] (ab271344)
    All lanes : Anti-Histone H2B (acetyl K20) antibody [RM235] (ab271344) at 0.5 µg/ml

    Lane 1 : Acid extracts of HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with sodium butyrate
    Lane 2 : Acid extracts of untreated HeLa cells

    Predicted band size: 14 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K20) antibody [RM235] (ab271344)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K20) antibody [RM235] (ab271344)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells treated with sodium butyrate stained for Histone H2B (acetyl K20) using ab271344 at 2 µg/ml (red). Actin filaments have been labeled with fluorescein phalloidin (green).

  • ELISA - Anti-Histone H2B (acetyl K20) antibody [RM235] (ab271344)
    ELISA - Anti-Histone H2B (acetyl K20) antibody [RM235] (ab271344)

    ab271344 specifically reacts to Histone H2B acetylated at Lysine 20 (K20ac). No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B. 

  • Anti-Histone H2B (acetyl K20) antibody [RM235] (ab271344)
    Anti-Histone H2B (acetyl K20) antibody [RM235] (ab271344)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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