Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H2A (phospho T120) antibody [EPR17492] - BSA and Azide free (ab249963)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17492] to Histone H2A (phospho T120) - BSA and Azide free
  • Suitable for: PepArr, ELISA, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Histone H2A (phospho T120) antibody [EPR17492] - BSA and Azide free
    See all Histone H2A primary antibodies

  • Description

    Rabbit monoclonal [EPR17492] to Histone H2A (phospho T120) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: PepArr, ELISA, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249963 is the carrier-free version of ab177391. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249963 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17492

  • Isotype

    IgG

Images

  • Western blot - Anti-Histone H2A (phospho T120) antibody [EPR17492] - BSA and Azide free (ab249963)
    Western blot - Anti-Histone H2A (phospho T120) antibody [EPR17492] - BSA and Azide free (ab249963)
    All lanes : Anti-Histone H2A (phospho T120) antibody [EPR17492] (ab177391) at 1/1000 dilution

    Lane 1 : Core histones purified by acid extraction precipitation from untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) cells
    Lane 2 : Core histones purified by acid extraction precipitation from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells treated with colcemid

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 14 kDa
    Observed band size: 14 kDa


    Exposure time: 1 second


    This data was developed using ab177391, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 2% BSA/TBST.

    This target is specifically expressed at M phase according to the literature (PMID: 19965387).

  • Peptide Array - Anti-Histone H2A (phospho T120) antibody [EPR17492] - BSA and Azide free (ab249963)
    Peptide Array - Anti-Histone H2A (phospho T120) antibody [EPR17492] - BSA and Azide free (ab249963)

    This data was developed using ab177391, the same antibody clone in a different buffer formulation.

    ab177391 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).

    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.

    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho T120) antibody [EPR17492] - BSA and Azide free (ab249963)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho T120) antibody [EPR17492] - BSA and Azide free (ab249963)

    This data was developed using ab177391, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Histone H2A (phospho T120) with ab177391 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadically nuclear staining on human colon [PMID: 19965387]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho T120) antibody [EPR17492] - BSA and Azide free (ab249963)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho T120) antibody [EPR17492] - BSA and Azide free (ab249963)

    This data was developed using ab177391, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Histone H2A (phospho T120) with ab177391 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadically nuclear staining on germinal center of human spleen [PMID: 19965387]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho T120) antibody [EPR17492] - BSA and Azide free (ab249963)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho T120) antibody [EPR17492] - BSA and Azide free (ab249963)
    This data was developed using ab177391, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human gastric tumor tissue labeling Histone H2A (phospho T120) with ab177391 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadically nuclear staining on tumor cells of human gastric tumor [PMID: 19965387]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • ELISA - Anti-Histone H2A (phospho T120) antibody [EPR17492] - BSA and Azide free (ab249963)
    ELISA - Anti-Histone H2A (phospho T120) antibody [EPR17492] - BSA and Azide free (ab249963)
    This data was developed using ab177391, the same antibody clone in a different buffer formulation.Direct ELISA analysis using ab177391 (0 to 1000 ng/ml) in peptide ELISA with H2A T120 phospho peptide, H2A unmodified peptide, H2A.XT120 phospho peptide and H2A.X unmodified peptide as antigens (at 1000 ng/ml concentration). An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as secondary antibody.
  • Anti-Histone H2A (phospho T120) antibody [EPR17492] - BSA and Azide free (ab249963)
    Anti-Histone H2A (phospho T120) antibody [EPR17492] - BSA and Azide free (ab249963)

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