Anti-Histone H2A-Bbd antibody (ab4175)
Key features and details
- Rabbit polyclonal to Histone H2A-Bbd
- Suitable for: ICC, WB
- Reacts with: Mouse, Chicken, Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H2A-Bbd antibody -
Description
Rabbit polyclonal to Histone H2A-Bbd -
Host species
Rabbit -
Specificity
Known to detect the recombinant form of human Histone H2A BBD. However, no reaction has yet been demonstrated against the endogenous protein in histone preparations. We believe that this is because of the low abundance of this variant in such preparations. -
Tested Applications & Species
See all applications and species dataApplication Species ICC MouseWB ChickenHuman
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Immunogen
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab4175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC MouseWB ChickenHumanApplication Abreviews Notes ICC Use a concentration of 3 µg/ml. Staining may not be specific for H2A.Bbd (see image).WB 1/500. Detects a band of approximately 12.5 kDa (predicted molecular weight: 12.5 kDa). As the WB shows, we can not find any reaction with any of the different histone extracts that we have used from different sources (calf, human, chicken). However, the antibody is very specific against the recombinant form of the protein.Notes ICC
Use a concentration of 3 µg/ml. Staining may not be specific for H2A.Bbd (see image).WB
1/500. Detects a band of approximately 12.5 kDa (predicted molecular weight: 12.5 kDa). As the WB shows, we can not find any reaction with any of the different histone extracts that we have used from different sources (calf, human, chicken). However, the antibody is very specific against the recombinant form of the protein.Target
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Function
Atypical histone H2A which can replace conventional H2A in some nucleosomes and is associated with active transcription and mRNA processing. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Nucleosomes containing this histone are less rigid and organize less DNA than canonical nucleosomes in vivo. They are enriched in actively transcribed genes and associate with the elongating form of RNA polymerase. They associate with spliceosome components and are required for mRNA splicing. May participate in spermatogenesis. -
Tissue specificity
Present in mature sperm. -
Sequence similarities
Belongs to the histone H2A family. -
Domain
The docking domain is responsible for the weaker heterodimerization with H2B. -
Cellular localization
Nucleus. Chromosome. Associated with the active X chromosome and with autosomes, while it is absent from the inactive X chromosome and excluded from Barr bodies. - Information by UniProt
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Database links
- Entrez Gene: 474381 Human
- Entrez Gene: 474382 Human
- Entrez Gene: 83740 Human
- Omim: 300445 Human
- SwissProt: P0C5Y9 Human
- SwissProt: P0C5Z0 Human
- SwissProt: 68231 Mouse
- SwissProt: Q9CQ70 Mouse
see all -
Alternative names
- H2A Barr body deficient antibody
- H2A Barr body-deficient antibody
- H2A histone family member B antibody
see all
Images
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Western blot - Anti-Histone H2A-Bbd antibody (ab4175)Juan Ausio
H2A-Bbd (ab4175)
CE= chicken erythrocyte (10 and 2 micrograms) , HE= HeLa cells (10 micrograms) and 2A*= recombinant H2ABbd (the numbers underneath the lanes are the number of nanograms loaded in each lane).
The primary antibody dilution was 1:500 and the secondary was 1:3000.As the WB shows, we can not find any reaction with any of the different histone extracts that we have used from different sources (calf, human, chicken). However, the antibody is very specific against the recombinant form of the protein.
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Immunocytochemistry - Anti-Histone H2A-Bbd antibody (ab4175)This image is courtesy of Petra Hajkova, University of CambridgeMouse embryonic fibroblasts (MEFs) were fixed in 4%PFA, permeabilised in PBS 1%BSA 0.1%TX-100 and incubated with ab4175 (anti-H2A.Bbd) antibody o/n. The antibody appears to show nucleolar staining. The antibody gave the same staining pattern in different cell types. The dilution used was 3ug/ml.
Protocols
Datasheets and documents
References (3)
ab4175 has been referenced in 3 publications.
- Wu BJ et al. Localization and expression of histone H2A variants during mouse oogenesis and preimplantation embryo development. Genet Mol Res 13:5929-39 (2014). PubMed: 25117351
- Nashun B et al. Changes in the nuclear deposition of histone H2A variants during pre-implantation development in mice. Development 137:3785-94 (2010). WB, ICC/IF ; Mouse . PubMed: 20943707
- Lee JH et al. Chromatin analysis of occluded genes. Hum Mol Genet 18:2567-74 (2009). ChIP ; Human . PubMed: 19380460
Images
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Western blot - Anti-Histone H2A-Bbd antibody (ab4175) Juan Ausio
H2A-Bbd (ab4175)
CE= chicken erythrocyte (10 and 2 micrograms) , HE= HeLa cells (10 micrograms) and 2A*= recombinant H2ABbd (the numbers underneath the lanes are the number of nanograms loaded in each lane).
The primary antibody dilution was 1:500 and the secondary was 1:3000.As the WB shows, we can not find any reaction with any of the different histone extracts that we have used from different sources (calf, human, chicken). However, the antibody is very specific against the recombinant form of the protein.
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Immunocytochemistry - Anti-Histone H2A-Bbd antibody (ab4175) This image is courtesy of Petra Hajkova, University of Cambridge
Mouse embryonic fibroblasts (MEFs) were fixed in 4%PFA, permeabilised in PBS 1%BSA 0.1%TX-100 and incubated with ab4175 (anti-H2A.Bbd) antibody o/n. The antibody appears to show nucleolar staining. The antibody gave the same staining pattern in different cell types. The dilution used was 3ug/ml.