Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H1.3 (phospho T17) + Histone H1.4 antibody [EPR18087] - BSA and Azide free (ab250946)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18087] to Histone H1.3 (phospho T17) + Histone H1.4 (phospho T17) - BSA and Azide free
  • Suitable for: PepArr, ICC, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Histone H1.3 (phospho T17) + Histone H1.4 antibody [EPR18087] - BSA and Azide free
    See all Histone H1.3+Histone H1.4 primary antibodies

  • Description

    Rabbit monoclonal [EPR18087] to Histone H1.3 (phospho T17) + Histone H1.4 (phospho T17) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: PepArr, ICC, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide aa 1-100 (phospho T17). The exact sequence is proprietary.
    Database link: P10412

  • General notes

    Ab250946 is the carrier-free version of ab188294. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250946 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Histone H1.3 (phospho T17) + Histone H1.4 antibody [EPR18087] - BSA and Azide free (ab250946)
    Western blot - Anti-Histone H1.3 (phospho T17) + Histone H1.4 antibody [EPR18087] - BSA and Azide free (ab250946)
    All lanes : Anti-Histone H1.3 (phospho T17) + Histone H1.4 (phospho T17) antibody [EPR18087] (ab188294) at 1/5000 dilution

    Lane 1 : Untreated HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) treated with Colcemid acid extraction lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 22 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    This data was developed using ab188294, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Histone H1.3 (phospho T17) + Histone H1.4 antibody [EPR18087] - BSA and Azide free (ab250946)
    Western blot - Anti-Histone H1.3 (phospho T17) + Histone H1.4 antibody [EPR18087] - BSA and Azide free (ab250946)
    All lanes : Anti-Histone H1.3 (phospho T17) + Histone H1.4 (phospho T17) antibody [EPR18087] (ab188294) at 1/5000 dilution

    Lane 1 : Untreated NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates
    Lane 2 : NIH/3T3 (Mouse embyro fibroblast cells) treated with 1.5μg/ml Colcemid for 12 hours whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 22 kDa
    Observed band size: 30 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab188294, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Peptide Array - Anti-Histone H1.3 (phospho T17) + Histone H1.4 antibody [EPR18087] - BSA and Azide free (ab250946)
    Peptide Array - Anti-Histone H1.3 (phospho T17) + Histone H1.4 antibody [EPR18087] - BSA and Azide free (ab250946)
    This data was developed using ab188294, the same antibody clone in a different buffer formulation.ab188294 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).

    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.

    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.3 (phospho T17) + Histone H1.4 antibody [EPR18087] - BSA and Azide free (ab250946)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.3 (phospho T17) + Histone H1.4 antibody [EPR18087] - BSA and Azide free (ab250946)
    This data was developed using ab188294, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Histone H1.3 (phospho T17) + Histone H1.4 (phospho T17) with ab188294 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on epithelial cells of Human colon is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.3 (phospho T17) + Histone H1.4 antibody [EPR18087] - BSA and Azide free (ab250946)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.3 (phospho T17) + Histone H1.4 antibody [EPR18087] - BSA and Azide free (ab250946)
    This data was developed using ab188294, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Histone H1.3 (phospho T17) + Histone H1.4 (phospho T17) with ab188294 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on epithelial cells of mouse colon is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.3 (phospho T17) + Histone H1.4 antibody [EPR18087] - BSA and Azide free (ab250946)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.3 (phospho T17) + Histone H1.4 antibody [EPR18087] - BSA and Azide free (ab250946)
    This data was developed using ab188294, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Histone H1.3 (phospho T17) + Histone H1.4 (phospho T17) with ab188294 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on epithelial cells of rat colon is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-Histone H1.3 (phospho T17) + Histone H1.4 antibody [EPR18087] - BSA and Azide free (ab250946)
    Immunocytochemistry - Anti-Histone H1.3 (phospho T17) + Histone H1.4 antibody [EPR18087] - BSA and Azide free (ab250946)
    This data was developed using ab188294, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Histone H1.3 (phospho T17) + Histone H1.4 (phospho T17) with ab188294 at 1/8000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).Confocal image showing nuclear staining on HeLa cell line.The nuclear counter stain is DAPI (blue).Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:
    -ve control 1: ab188294 at 1/8000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
  • Anti-Histone H1.3 (phospho T17) + Histone H1.4 antibody [EPR18087] - BSA and Azide free (ab250946)
    Anti-Histone H1.3 (phospho T17) + Histone H1.4 antibody [EPR18087] - BSA and Azide free (ab250946)

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