Anti-Hhip antibody (ab39208)
Key features and details
- Rabbit polyclonal to Hhip
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-Hhip antibody
See all Hhip primary antibodies -
Description
Rabbit polyclonal to Hhip -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Hhip.
Read Abcam's proprietary immunogen policy (Peptide available as ab39947.) -
Positive control
- This antibody gave a positive signal in the following lysates: HeLa Whole Cell; Mouse Heart; Mouse Liver; Mouse Pancreas.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab39208 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications CowApplication Abreviews Notes WB (1) Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 79 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 79 kDa).Target
-
Function
Modulates hedgehog signaling in several cell types including brain and lung through direct interaction with members of the hedgehog family. -
Tissue specificity
Widely expressed in fetal and adult tissues. Highest expression in adult heart, liver and pancreas, and in fetal kidney. -
Sequence similarities
Belongs to the HHIP family.
Contains 2 EGF-like domains. -
Domain
A flexible loop interacts with the SHH zinc binding site and contributes to zinc binding. -
Cellular localization
Cytoplasm and Cell membrane. Secreted. The last 22 C-terminal amino acids may participate in cell membrane attachment. - Information by UniProt
-
Database links
- Entrez Gene: 540830 Cow
- Entrez Gene: 64399 Human
- Entrez Gene: 15245 Mouse
- Omim: 606178 Human
- SwissProt: Q96QV1 Human
- SwissProt: Q7TN16 Mouse
- Unigene: 507991 Human
- Unigene: 254493 Mouse
-
Alternative names
- FLJ20992 antibody
- FLJ90230 antibody
- Hedgehog interacting protein antibody
see all
Images
-
Western blot - Anti-Hhip antibody (ab39208)All lanes : Anti-Hhip antibody (ab39208) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Heart (Mouse) Tissue Lysate
Lane 3 : Liver (Mouse) Tissue Lysate
Lane 4 : Pancreas (Mouse) Tissue Lysate
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human Hhip peptide (ab39947) at 1 µg/ml
Lane 6 : Heart (Mouse) Tissue Lysate with Human Hhip peptide (ab39947) at 1 µg/ml
Lane 7 : Liver (Mouse) Tissue Lysate with Human Hhip peptide (ab39947) at 1 µg/ml
Lane 8 : Pancreas (Mouse) Tissue Lysate with Human Hhip peptide (ab39947) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 79 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 45 kDa, 47 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
References (4)
ab39208 has been referenced in 4 publications.
- Lee BNR et al. Hedgehog-Interacting Protein (HIP) Regulates Apoptosis Evasion and Angiogenic Function of Late Endothelial Progenitor Cells. Sci Rep 7:12449 (2017). WB ; Mouse . PubMed: 28963460
- Wang C et al. EZH2 Mediates epigenetic silencing of neuroblastoma suppressor genes CASZ1, CLU, RUNX3, and NGFR. Cancer Res 72:315-24 (2012). PubMed: 22068036
- Zhou X et al. Identification of a chronic obstructive pulmonary disease genetic determinant that regulates HHIP. Hum Mol Genet 21:1325-35 (2012). WB ; Human . PubMed: 22140090
- Angeloni NL et al. The role of hedgehog-interacting protein in maintaining cavernous nerve integrity and adult penile morphology. J Sex Med 6:2480-93 (2009). IHC-Fr ; Rat . PubMed: 19515211
Images
-
Western blot - Anti-Hhip antibody (ab39208)All lanes : Anti-Hhip antibody (ab39208) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Heart (Mouse) Tissue Lysate
Lane 3 : Liver (Mouse) Tissue Lysate
Lane 4 : Pancreas (Mouse) Tissue Lysate
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human Hhip peptide (ab39947) at 1 µg/ml
Lane 6 : Heart (Mouse) Tissue Lysate with Human Hhip peptide (ab39947) at 1 µg/ml
Lane 7 : Liver (Mouse) Tissue Lysate with Human Hhip peptide (ab39947) at 1 µg/ml
Lane 8 : Pancreas (Mouse) Tissue Lysate with Human Hhip peptide (ab39947) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 79 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 45 kDa, 47 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
