Anti-Hepatitis C Virus E2 antibody (ab20044)
Key features and details
- Goat polyclonal to Hepatitis C Virus E2
- Suitable for: ELISA, WB
- Isotype: IgG
Overview
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Product name
Anti-Hepatitis C Virus E2 antibody
See all Hepatitis C Virus E2 primary antibodies -
Description
Goat polyclonal to Hepatitis C Virus E2 -
Host species
Goat -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with Hepatitis C Virus. Not yet tested in other species. -
Immunogen
Recombinant full length E2 (genotype 1a) protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Purification notes
>95% pure. Sodium sulfate precipitation and ion-exchange chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ELISA - Anti-Hepatitis C Virus E2 antibody (ab20044) Image from Takayama H et al., PLoS One. 2008 Jun 4;3(6):e2314. doi: 10.1371/journal.pone.0002314.; Fig 5.; June 4, 2008, PLoS ONE 3(6): e2314.ELISA analysis of Hepatitis C Virus E2 binding to CD81 receptor, using ab20044 to detect Hepatitis C Virus E2 binding.
A 96-well plate was coated with 100 ng of rho-1D4 antibody. Following overnight incubation at 4°C, the unbound antibody was washed away. The wells were blocked with blocking solution for 2 hours. 100 ng of purified CD81 was added to the wells along with E2 glycoprotein (0.4 to 20 nM) and incubated for 2 hours. ab20044 anti-Hepatitis C Virus E2 antibody (1/2000 dilution) and secondary antibody with alkaline phosphatase conjugate (1/1000 dilution) were added and incubated for 2 hours. Absorbance was measured at 495 nm by a plate-reader.