Antibodies, Proteins, Kits and Reagents for Life Science

271 382 ₸ Product size

100 µg 271 382 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349)

Key features and details

Mouse monoclonal [1F12-A6] to Heme Oxygenase 1
Suitable for: IHC-P, ICC/IF, WB
Reacts with: Mouse, Human
Isotype: IgG1

Product name

Anti-Heme Oxygenase 1 antibody [1F12-A6]
See all Heme Oxygenase 1 primary antibodies
Description

Mouse monoclonal [1F12-A6] to Heme Oxygenase 1
Host species

Mouse

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Mouse
WB	Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human Heme Oxygenase 1 aa 1-100.
Database link: P09601

Run BLAST with Run BLAST with
Positive control
- ICC/IF: HeLa and HaCaT cells. IHC: Mouse back skin. WB: HeLa cell lysate.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein G purified
Clonality

Monoclonal
Clone number

1F12-A6
Isotype

IgG1
Light chain type

kappa
Research areas

Western blot - Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349)

Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 15 µg

Secondary
Sheep Anti-Mouse IgG: HRP

Predicted band size: 33 kDa

Blocking: 1.5% BSA for 30 minutes at RT.
Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349)

2% formaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Heme Oxygenase 1 (green) using ab223349 at a 1/100 dilution in ICC/IF. Secondary: FITC Goat Anti-Mouse at 1/200 dilution for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 dilution for 2 hours at RT.

(A) DAPI nuclear stain (B) ab223349 (C) Merge.
Immunohistochemistry paraffin embedded sections - Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349)

Bouin's fixative, paraffin embedded mouse back skin tissue stained for Heme Oxygenase 1 (green) with ab223349 at a 1/100 dilution in immunohistochemical analysis. Secondary Antibody: FITC Goat Anti-Mouse at 1/50 for 1 hour at RT.
Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349)

2% formaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Heme Oxygenase 1 (yellow) using ab223349 at a 1/100 dilution in ICC/IF. Secondary: R-PE Goat Anti-Mouse at 1/200 dilution for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 dilution for 2 hours at RT.

(A) DAPI nuclear stain (B) ab223349 (C) Merge.
Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349)

100% methanol fixed HaCaT (human keratinocyte cell line) cells stained for Heme Oxygenase 1 (green) using ab223349 at a 1/100 dilution in ICC/IF. Secondary: FITC Goat Anti-Mouse at 1/50 dilution for 1 hour at RT.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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