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Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349)

Key features and details

  • Mouse monoclonal [1F12-A6] to Heme Oxygenase 1
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Heme Oxygenase 1 antibody [1F12-A6]
    See all Heme Oxygenase 1 primary antibodies

  • Description

    Mouse monoclonal [1F12-A6] to Heme Oxygenase 1

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human Heme Oxygenase 1 aa 1-100.
    Database link: P09601

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • ICC/IF: HeLa and HaCaT cells. IHC: Mouse back skin. WB: HeLa cell lysate.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349)
    Western blot - Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349)
    Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 15 µg

    Secondary
    Sheep Anti-Mouse IgG: HRP

    Predicted band size: 33 kDa



    Blocking: 1.5% BSA for 30 minutes at RT.

  • Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349)
    Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349)

    2% formaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Heme Oxygenase 1 (green) using ab223349 at a 1/100 dilution in ICC/IF. Secondary: FITC Goat Anti-Mouse at 1/200 dilution for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 dilution for 2 hours at RT.

    (A) DAPI nuclear stain (B) ab223349 (C) Merge.

     

  • Immunohistochemistry paraffin embedded sections - Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349)
    Immunohistochemistry paraffin embedded sections - Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349)

    Bouin's fixative, paraffin embedded mouse back skin tissue stained for Heme Oxygenase 1 (green) with ab223349 at a 1/100 dilution in immunohistochemical analysis. Secondary Antibody: FITC Goat Anti-Mouse at 1/50 for 1 hour at RT. 

  • Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349)
    Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349)

    2% formaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Heme Oxygenase 1 (yellow) using ab223349 at a 1/100 dilution in ICC/IF. Secondary: R-PE Goat Anti-Mouse at 1/200 dilution for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 dilution for 2 hours at RT.

    (A) DAPI nuclear stain (B) ab223349 (C) Merge.

  • Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349)
    Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349)

    100% methanol fixed HaCaT (human keratinocyte cell line) cells stained for Heme Oxygenase 1 (green) using ab223349 at a 1/100 dilution in ICC/IF. Secondary: FITC Goat Anti-Mouse at 1/50 dilution for 1 hour at RT.

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