All lanes : Anti-HDGF antibody [EPR7898] (ab128921) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : SKBR-3 cell lysate
Lane 4 : ECV-304 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP-conjugated at 1/2000 dilution

Predicted band size: 27 kDa

This data was developed using ab128921, the same antibody clone in a different buffer formulation.

This data was developed using ab128921, the same antibody clone in a different buffer formulation.ab128921 staining HDGF in MCF-7 (human breast carcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain and the negative control was PBS only.

This data was developed using ab128921, the same antibody clone in a different buffer formulation.Overlay histogram showing HeLa cells stained with ab128921 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab128921, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1?g/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

This data was developed using ab128921, the same antibody clone in a different buffer formulation.ab128921, at a 1/100 dilution, staining HDGF in paraffin embedded Human hepatocellular carcinoma tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

This data was developed using ab128921, the same antibody clone in a different buffer formulation.ab128921, at a 1/100 dilution, staining HDGF in paraffin embedded Human colon tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.