Anti-HDAC6 antibody - N-terminal (ab226959)
Key features and details
- Rabbit polyclonal to HDAC6 - N-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-HDAC6 antibody - N-terminal
See all HDAC6 primary antibodies -
Description
Rabbit polyclonal to HDAC6 - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
-
Immunogen
Synthetic peptide within Human HDAC6 (N terminal). The exact sequence is proprietary. Carrier-protein conjugated.
Database link: Q9UBN7 -
Positive control
- ICC/IF: HeLa cells. WB: HDAC6 transfected HEK-293T whole cell extract.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-HDAC6 antibody - N-terminal (ab226959)
Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HDAC6 (green) using ab226959 at 1/500 dilution in ICC/IF. Counterstain: alpha-tubulin filaments were labeled with an anti-alpha Tubulin antibody (red) at 1/2000.
-
Western blot - Anti-HDAC6 antibody - N-terminal (ab226959)All lanes : Anti-HDAC6 antibody - N-terminal (ab226959) at 1/5000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with HDAC6 (3xFlag-tag), whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 131 kDa5% SDS-PAGE gel.
