Anti-HDAC6 antibody [3D2] (ab253034)
![Anti-HDAC6 antibody [3D2] (ab253034)](https://www.abcam.com/ps/products/253/ab253034/Images/ab253034-343573-Mouse-monoclonal-3D2-to-HDAC6-immunocytochemistry-hek293t-shsy5y-human.jpg "Anti-HDAC6 antibody [3D2] (ab253034)")
Key features and details
- Mouse monoclonal [3D2] to HDAC6
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HDAC6 antibody [3D2]
See all HDAC6 primary antibodies -
Description
Mouse monoclonal [3D2] to HDAC6 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human HDAC6.
Database link: Q9UBN7 -
Epitope
D2 domain of HDAC6 (region amino acids 471-481). -
Positive control
- ICC/IF: HEK-293T and SH-SY5Y cells. WB: HEK-293T lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from hybridoma culture supernatant. -
Clonality
Monoclonal -
Clone number
3D2 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-HDAC6 antibody [3D2] (ab253034)
Formaldehyde-fixed, Triton permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) (Panel A) and SH-SY5Y (Human neuroblastoma cell line from bone marrow) (Panel B) cells labeling HDAC6 using ab253034 at 25 µg/ml, overnight (green) followed by Alexa Fluor® 488 secondary antibody and Alexa Fluor® 647 secondary antibody respectively, in ICC/IF. Nuclear probe is visualized in blue (DAPI). Tubulin is visualized in Red.
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![Western blot - Anti-HDAC6 antibody [3D2] (ab253034)](https://www.abcam.com/ps/products/253/ab253034/Images/ab253034-343572-Mouse-monoclonal-3D2-to-HDAC6-western-blot-hek293t-human.jpg)
Western blot - Anti-HDAC6 antibody [3D2] (ab253034)Anti-HDAC6 antibody [3D2] (ab253034) at 1 µg/ml + HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Secondary
HRP-conjugated anti-mouse IgG
Predicted band size: 131 kDaMembrane was blocked with 5% milk.
Reducing conditions using PBS/0.05% Tween-20 buffer.