Anti-HDAC4 (phospho S632) antibody (ab39408)
Key features and details
- Rabbit polyclonal to HDAC4 (phospho S632)
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-HDAC4 (phospho S632) antibody
See all HDAC4 primary antibodies -
Description
Rabbit polyclonal to HDAC4 (phospho S632) -
Host species
Rabbit -
Specificity
ab39408 detects endogenous levels of HDAC4 only when phosphorylated at serine 632. -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic phosphopeptide derived from human HDAC4 around the phosphorylation site of serine 632 (A-Q-SP-S-P)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HDAC4 (phospho S632) antibody (ab39408)All lanes : Anti-HDAC4 (phospho S632) antibody (ab39408) at 1/500 dilution
Lane 1 : Extract from Jurkat cells (5-30ug of total protein)
Lane 2 : Extract from Jurkat cells treated with Calyculin A (5-30ug of total protein)
Predicted band size: 119 kDa
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Western blot - Anti-HDAC4 (phospho S632) antibody (ab39408) Image from Evankovich J et al, J Biol Chem. 2010 Dec 17;285(51):39888-97. Epub 2010 Oct 11, Fig 4.20 µg of nuclear lysate prepared from mouse liver was diluted in SDS buffer and run on SDS-PAGE gel. Samples were transferred to nitrocellulose overnight, and blocked with 5% BSA for 8 hours. They were then incubated overnight with primary antibodies, which included an anti-HDAC1, 1/1000 and ab39408, 1/1000. After washing, membranes were incubated with horseradish peroxidase-coupled rabbit or mouse secondary antibodies (1/5000 to 1/10,000) in 5% milk for 1 hour and developed with a Super Signal West Pico chemiluminescent kit. -
Western blot - Anti-HDAC4 (phospho S632) antibody (ab39408) Image courtesy of an anonymous Abreview.All lanes : Anti-HDAC4 (phospho S632) antibody (ab39408) at 1/500 dilution
Lane 1 : Lysate from murine heart tissue 1
Lane 2 : Lysate from murine heart tissue 2
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRp conjugated goat anti-rabbit polyclonal at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 119 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
