Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HDAC11/HD11 antibody [EPR23540-414] - BSA and Azide free (ab274389)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23540-414] to HDAC11/HD11 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-HDAC11/HD11 antibody [EPR23540-414] - BSA and Azide free
    See all HDAC11/HD11 primary antibodies

  • Description

    Rabbit monoclonal [EPR23540-414] to HDAC11/HD11 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: T-47D, SK-OV-3 and MCF7 whole cell lysates; Human brain tissue lysate. IP: Human brain tissue lysate; T-47D whole cell lysate.

  • General notes

    ab274389 is the carrier-free version of ab246512. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-HDAC11/HD11 antibody [EPR23540-414] - BSA and Azide free (ab274389)
    Western blot - Anti-HDAC11/HD11 antibody [EPR23540-414] - BSA and Azide free (ab274389)
    All lanes : Anti-HDAC11/HD11 antibody [EPR23540-414] (ab246512) at 1/1000 dilution

    Lane 1 : Human brain tissue lysate
    Lane 2 : Human spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))
    Lane 2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: human spleen (PMID:11948178).

    Exposure time: 3 minutes

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246512)

  • Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR23540-414] - BSA and Azide free (ab274389)
    Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR23540-414] - BSA and Azide free (ab274389)

    HDAC11/HD11 was immunoprecipitated from 0.35 mg Human brain tissue lysate with ab246512 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246512 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Human brain tissue lysate 10 ug.

    Lane 2: ab246512 IP in Human brain tissue lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246512 in human brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 50 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246512).

  • Western blot - Anti-HDAC11/HD11 antibody [EPR23540-414] - BSA and Azide free (ab274389)
    Western blot - Anti-HDAC11/HD11 antibody [EPR23540-414] - BSA and Azide free (ab274389)
    All lanes : Anti-HDAC11/HD11 antibody [EPR23540-414] (ab246512) at 1/1000 dilution

    Lane 1 : T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate
    Lane 2 : SK-OV-3 (human ovarian cancer epithelial cell), whole cell lysate
    Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
    Lanes 2-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246512)

  • Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR23540-414] - BSA and Azide free (ab274389)
    Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR23540-414] - BSA and Azide free (ab274389)

    HDAC11/HD11 was immunoprecipitated from 0.35 mg T-47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate with ab246512 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246512 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: T-47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate 10 ug.

    Lane 2: ab246512 IP in T-47D whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246512 in T-47D whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds

    This blot was developed using a higher sensitivity ECL substrate.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246512).

  • Anti-HDAC11/HD11 antibody [EPR23540-414] - BSA and Azide free (ab274389)
    Anti-HDAC11/HD11 antibody [EPR23540-414] - BSA and Azide free (ab274389)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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