Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HCN1 antibody [7C3] - BSA and Azide free (ab255791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [7C3] to HCN1 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-HCN1 antibody [7C3] - BSA and Azide free
    See all HCN1 primary antibodies

  • Description

    Rat monoclonal [7C3] to HCN1 - BSA and Azide free

  • Host species

    Rat

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • WB: Human, mouse and rat brain tissue lysate. IHC-P: Human, rat and mouse cerebrum tissue. Mouse and rat retina tissue.

  • General notes

    ab255791 is the carrier-free version of ab252835.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] - BSA and Azide free (ab255791)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] - BSA and Azide free (ab255791)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling HCN1 with ab252835 at 0.065µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on rat cerebrum tissue is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252835).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] - BSA and Azide free (ab255791)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] - BSA and Azide free (ab255791)

    Immunohistochemical analysis of paraffin-embedded rat retina tissue labeling HCN1 with ab252835 at 0.065µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on rat retina tissue is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252835).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] - BSA and Azide free (ab255791)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] - BSA and Azide free (ab255791)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling HCN1 with ab252835 at 0.065µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on mouse cerebrum tissue is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252835).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] - BSA and Azide free (ab255791)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] - BSA and Azide free (ab255791)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling HCN1 with ab252835 at 0.065µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on human esophagus tissue is observed. The section was incubated with ab252835 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252835).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] - BSA and Azide free (ab255791)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] - BSA and Azide free (ab255791)

    Immunohistochemical analysis of paraffin-embedded mouse retina tissue labeling HCN1 with ab252835 at 0.065µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on mouse retina tissue is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252835).

  • Western blot - Anti-HCN1 antibody [7C3] - BSA and Azide free (ab255791)
    Western blot - Anti-HCN1 antibody [7C3] - BSA and Azide free (ab255791)
    All lanes : Anti-HCN1 antibody [7C3] (ab252835) at 0.326 µg/ml

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

    Predicted band size: 99 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 secs.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252835).

  • Western blot - Anti-HCN1 antibody [7C3] - BSA and Azide free (ab255791)
    Western blot - Anti-HCN1 antibody [7C3] - BSA and Azide free (ab255791)
    All lanes : Anti-HCN1 antibody [7C3] (ab252835) at 0.326 µg/ml

    Lane 1 : Human brain tissue lysate
    Lane 2 : HEK-293 (human embryonic kidney epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

    Predicted band size: 99 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 5 secs.

    The negative control and the molecular weight of HCN1 observed is consistent with what has been described in the literature (PMID: 12786975).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252835).

  • Anti-HCN1 antibody [7C3] - BSA and Azide free (ab255791)
    Anti-HCN1 antibody [7C3] - BSA and Azide free (ab255791)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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