Anti-HARS antibody (ab246996)
Key features and details
- Rabbit polyclonal to HARS
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-HARS antibody
See all HARS primary antibodies -
Description
Rabbit polyclonal to HARS -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Orangutan
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Immunogen
Recombinant fragment corresponding to Human HARS aa 1-100.
Database link: P12081 -
Positive control
- WB: Caco-2, NIH/3T3 and NBT-II whole cell lysates. IHC-P: Human skin, cerebral cortex, cerebellum and kidney tissue. ICC/IF: A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-HARS antibody (ab246996)
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for HARS (green) using ab246996 at 4 μg/ml in ICC/IF.
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Western blot - Anti-HARS antibody (ab246996)All lanes : Anti-HARS antibody (ab246996) at 0.4 µg/ml
Lane 1 : Caco-2 (human colorectal adenocarcinoma cell line) transfected with control siRNA, whole cell lysate
Lane 2 : Caco-2 (human colorectal adenocarcinoma cell line) transfected with target-specific siRNA #1, whole cell lysate
Lane 3 : Caco-2 (human colorectal adenocarcinoma cell line) transfected with target-specific siRNA #2, whole cell lysate
Predicted band size: 57 kDaLoading control: Anti-GAPDH.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HARS antibody (ab246996)Paraffin-embedded human kidney tissue stained for HARS using ab246996 at 1/500 dilution in immunohistochemical analysis. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HARS antibody (ab246996)Paraffin-embedded human cerebellum tissue stained for HARS using ab246996 at 1/500 dilution in immunohistochemical analysis. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HARS antibody (ab246996)Paraffin-embedded human cerebral cortex tissue stained for HARS using ab246996 at 1/500 dilution in immunohistochemical analysis. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HARS antibody (ab246996)Paraffin-embedded human skin tissue stained for HARS using ab246996 at 1/500 dilution in immunohistochemical analysis. -
Western blot - Anti-HARS antibody (ab246996)All lanes : Anti-HARS antibody (ab246996) at 0.4 µg
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : NBT-II (rat Wistar bladder tumor cell line) whole cell lysate
Predicted band size: 57 kDa