Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GUCY1B3 antibody [EPR8822] - BSA and Azide free (ab232491)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8822] to GUCY1B3 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GUCY1B3 antibody [EPR8822] - BSA and Azide free
    See all GUCY1B3 primary antibodies

  • Description

    Rabbit monoclonal [EPR8822] to GUCY1B3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human kidney tissue.

  • General notes

    ab232491 is the carrier-free version of ab154841.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR8822

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] - BSA and Azide free (ab232491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] - BSA and Azide free (ab232491)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, labeling GUCY1B3 using unpurified ab154841 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154841).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] - BSA and Azide free (ab232491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] - BSA and Azide free (ab232491)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human thyroid gland carcinoma tissue, labeling GUCY1B3 using unpurified ab154841 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154841).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] - BSA and Azide free (ab232491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] - BSA and Azide free (ab232491)

    Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using unpurified ab154841 showing +ve staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154841).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] - BSA and Azide free (ab232491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] - BSA and Azide free (ab232491)

    Immunohistochemical analysis of paraffin embedded Human heart tissue using unpurified ab154841 showing +ve staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154841).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] - BSA and Azide free (ab232491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] - BSA and Azide free (ab232491)

    Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue using unpurified ab154841 showing +ve staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154841).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] - BSA and Azide free (ab232491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] - BSA and Azide free (ab232491)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling GUCY1B3 with purified ab154841 at 1:400 dilution (2.54 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154841).

  • Anti-GUCY1B3 antibody [EPR8822] - BSA and Azide free (ab232491)
    Anti-GUCY1B3 antibody [EPR8822] - BSA and Azide free (ab232491)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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