Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GSTT1 antibody [EPR19283] (ab199337)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19283] to GSTT1
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GSTT1 antibody [EPR19283]
    See all GSTT1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19283] to GSTT1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal kidney and fetal liver lysates; HepG2 whole cell lysate; Mouse lung and liver lysates; Rat lung and liver lysates. IP: HepG2 whole cell lysate.

  • General notes

     

     

     This product was previously labelled as Glutathione S Transferase theta 1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19283

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-GSTT1 antibody [EPR19283] (ab199337)
    Western blot - Anti-GSTT1 antibody [EPR19283] (ab199337)
    All lanes : Anti-GSTT1 antibody [EPR19283] (ab199337) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 27 kDa
    Observed band size: 24 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Jurkat doesn't express GSTT1. The expression profile observed is consistent with what has been described in the literature (PMID: 24586676).

  • Western blot - Anti-GSTT1 antibody [EPR19283] (ab199337)
    Western blot - Anti-GSTT1 antibody [EPR19283] (ab199337)
    Anti-GSTT1 antibody [EPR19283] (ab199337) at 1/1000 dilution + Human fetal liver lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 27 kDa
    Observed band size: 24 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-GSTT1 antibody [EPR19283] (ab199337)
    Western blot - Anti-GSTT1 antibody [EPR19283] (ab199337)
    All lanes : Anti-GSTT1 antibody [EPR19283] (ab199337) at 1/1000 dilution

    Lane 1 : Mouse lung lysate
    Lane 2 : Rat lung lysate
    Lane 3 : Mouse liver lysate
    Lane 4 : Rat liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 27 kDa
    Observed band size: 24 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1 and 2: 3 minutes; Lane 3: 5 seconds; Lane 4: 30 seconds.

  • Immunoprecipitation - Anti-GSTT1 antibody [EPR19283] (ab199337)
    Immunoprecipitation - Anti-GSTT1 antibody [EPR19283] (ab199337)

    GSTT1 was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab199337 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab199337 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
    Lane 1: HepG2 whole cell lysate, 10µg (Input).
    Lane 2: ab199337 IP in HepG2 whole cell lysate.
    Lane 3: Rabbit IgG,monoclonal [EPR25A] -Isotype Control (ab172730) instead of ab199337 in HepG2 whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 minutes.

  • Anti-GSTT1 antibody [EPR19283] (ab199337)
    Anti-GSTT1 antibody [EPR19283] (ab199337)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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