Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GRSF1 antibody [EPR16679] (ab194358)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16679] to GRSF1
  • Suitable for: Flow Cyt, IHC-P, WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-GRSF1 antibody [EPR16679]
    See all GRSF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR16679] to GRSF1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, HepG2, 293 and Jurkat whole cell lysate (ab7899); Human liver tissue; HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16679

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-GRSF1 antibody [EPR16679] (ab194358)
    Western blot - Anti-GRSF1 antibody [EPR16679] (ab194358)
    All lanes : Anti-GRSF1 antibody [EPR16679] (ab194358) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRSF1 antibody [EPR16679] (ab194358)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRSF1 antibody [EPR16679] (ab194358)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling GRSF1 with ab194358 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-GRSF1 antibody [EPR16679] (ab194358)
    Immunocytochemistry/ Immunofluorescence - Anti-GRSF1 antibody [EPR16679] (ab194358)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling GRSF1 with ab194358 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution. The nuclear counter stain is DAPI (blue).

  • Flow Cytometry - Anti-GRSF1 antibody [EPR16679] (ab194358)
    Flow Cytometry - Anti-GRSF1 antibody [EPR16679] (ab194358)

    Flow cytometry analysis of HeLa cells labelling GRSF1 (red) with purified ab194358 at dilution of 1/250. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

  • Anti-GRSF1 antibody [EPR16679] (ab194358)
    Anti-GRSF1 antibody [EPR16679] (ab194358)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-GRSF1 antibody [EPR16679] (ab194358)

© 2021 Astana Biomed Group LLP. All rights reserved.