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Signal Transduction Protein Trafficking Chaperones Other Chaperones

Anti-PFDN5 antibody [EPR7755] (ab129116)

Price and availability

241 228 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-PFDN5 antibody [EPR7755] (ab129116)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7755] to PFDN5
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-PFDN5 antibody [EPR7755]
    See all PFDN5 primary antibodies
  • Description

    Rabbit monoclonal [EPR7755] to PFDN5
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to residues in Human PFDN5 (Q99471).

  • Positive control

    • Human colon carcinoma tissue; 293T, HeLa, HepG2, fetal brain, fetal heart, fetal kidney, fetal muscle, Human spleen, NIH/3T3 and F9 cell lysates.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7755
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Other Chaperones
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors

Images

  • Western blot - Anti-PFDN5 antibody [EPR7755] (ab129116)
    Western blot - Anti-PFDN5 antibody [EPR7755] (ab129116)
    All lanes : Anti-PFDN5 antibody [EPR7755] (ab129116) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Fetal brain lysate
    Lane 4 : Fetal heart lysate
    Lane 5 : Fetal kidney lysate
    Lane 6 : Human spleen lysate
    Lane 7 : HepG2 cell lysate
    Lane 8 : NIH/3T3 lysate
    Lane 9 : F9 cell lysate
    Lane 10 : Fetal muscle lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 17 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFDN5 antibody [EPR7755] (ab129116)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFDN5 antibody [EPR7755] (ab129116)

    ab129116, at a 1/100 dilution, staining PFDN5 in paraffin-embedded Human colon carcinoma tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-PFDN5 antibody [EPR7755] (ab129116)
    Anti-PFDN5 antibody [EPR7755] (ab129116)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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