Anti-GRPR antibody (ab39883)
Key features and details
- Rabbit polyclonal to GRPR
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GRPR antibody
See all GRPR primary antibodies -
Description
Rabbit polyclonal to GRPR -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human GRPR.
Read Abcam's proprietary immunogen policy (Peptide available as ab39961.)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab39883 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatChickenApplication Abreviews Notes WB (1) Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 43 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 43 kDa).Target
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Function
Receptor for gastrin releasing peptide (GRP). This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. -
Tissue specificity
Highly expressed in pancreas. Also expressed in stomach, adrenal cortex and brain. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2925 Human
- Entrez Gene: 14829 Mouse
- Entrez Gene: 24938 Rat
- Omim: 305670 Human
- SwissProt: P30550 Human
- SwissProt: P21729 Mouse
- SwissProt: P52500 Rat
- Unigene: 567282 Human
see all -
Alternative names
- BB2 antibody
- Bombesin BB2 receptor antibody
- bombesin receptor 2 antibody
see all
Images
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Western blot - Anti-GRPR antibody (ab39883)All lanes : Anti-GRPR antibody (ab39883) at 1 µg/ml
Lane 1 : Brain (Human) Membrane Lysate
Lane 2 : MDA MB 361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 :Caco-2 whole cell lysate (ab3950)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
This protein is known to be glycosylated which could explain the difference between the predicted molecular weight of 43kDa according to Swiss Prot and the observed molecular weight of 53kDa.
Datasheets and documents
References (5)
ab39883 has been referenced in 5 publications.
- Acton D et al. Spinal Neuropeptide Y1 Receptor-Expressing Neurons Form an Essential Excitatory Pathway for Mechanical Itch. Cell Rep 28:625-639.e6 (2019). PubMed: 31315043
- Pu F et al. GRPR-targeted Protein Contrast Agents for Molecular Imaging of Receptor Expression in Cancers by MRI. Sci Rep 5:16214 (2015). PubMed: 26577829
- Pereira PJ et al. GRPR/PI3K?: Partners in Central Transmission of Itch. J Neurosci 35:16272-81 (2015). PubMed: 26658875
- Santos J et al. Uncovering potential downstream targets of oncogenic GRPR overexpression in prostate carcinomas harboring ETS rearrangements. Oncoscience 2:497-507 (2015). WB . PubMed: 26097883
- Tattersall M et al. Expression of gastrin-releasing peptide is increased by prolonged stretch of human myometrium, and antagonists of its receptor inhibit contractility. J Physiol 590:2081-93 (2012). WB ; Human . PubMed: 22411014
Images
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Western blot - Anti-GRPR antibody (ab39883)All lanes : Anti-GRPR antibody (ab39883) at 1 µg/ml
Lane 1 : Brain (Human) Membrane Lysate
Lane 2 : MDA MB 361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 :Caco-2 whole cell lysate (ab3950)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
This protein is known to be glycosylated which could explain the difference between the predicted molecular weight of 43kDa according to Swiss Prot and the observed molecular weight of 53kDa.
