Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GRK3 antibody [EPR2041(2)] (ab109303)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2041(2)] to GRK3
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-GRK3 antibody [EPR2041(2)]
    See all GRK3 primary antibodies

  • Description

    Rabbit monoclonal [EPR2041(2)] to GRK3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: human hepatocellular carcinoma and brain tissue. ICC/IF: Jurkat cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK3 antibody [EPR2041(2)] (ab109303)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK3 antibody [EPR2041(2)] (ab109303)
    Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling GRK3 with Purified ab109303 at 1:50 dilution (4.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK3 antibody [EPR2041(2)] (ab109303)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK3 antibody [EPR2041(2)] (ab109303)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue sections labeling GRK3 with Purified ab109303 at 1:500 dilution (0.46 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-GRK3 antibody [EPR2041(2)] (ab109303)
    Western blot - Anti-GRK3 antibody [EPR2041(2)] (ab109303)
    All lanes : Anti-GRK3 antibody [EPR2041(2)] (ab109303) at 1/10000 dilution (Purified)

    Lane 1 : Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysates
    Lane 2 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 80 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK3 antibody [EPR2041(2)] (ab109303)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK3 antibody [EPR2041(2)] (ab109303)

    ab109303, at 1/100 dilution, staining GRK3 in Human brain tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-GRK3 antibody [EPR2041(2)] (ab109303)
    Anti-GRK3 antibody [EPR2041(2)] (ab109303)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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