Anti-GRK3 antibody (ab38294)
Key features and details
- Rabbit polyclonal to GRK3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-GRK3 antibody
See all GRK3 primary antibodies -
Description
Rabbit polyclonal to GRK3 -
Host species
Rabbit -
Specificity
Targets C terminal of GRK3. The antibody might crossreact with GRK2 (immunogen sequence is 100% homolog with GRK2), but we do not have any testing data showing cross reactivity with GRK2. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Mouse
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Immunogen
Synthetic peptide conjugated to KLH and selected from the C terminal region of GRK3 (Human)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
This antibody was eluted out with both high and low pH buffers and neutralised immediately after elution then followed by dialysis against PBS -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GRK3 antibody (ab38294)Anti-GRK3 antibody (ab38294) + Mouse heart tissue lysate
Predicted band size: 80 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK3 antibody (ab38294)IHC image of ab38294 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab38294, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
