Anti-GPM6B antibody (ab92988)
Key features and details
- Rabbit polyclonal to GPM6B
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-GPM6B antibody
See all GPM6B primary antibodies -
Description
Rabbit polyclonal to GPM6B -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Cow, Human, Orangutan
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Immunogen
Synthetic peptide corresponding to Human GPM6B aa 200 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab104387) -
Positive control
- This antibody gave a positive signal in Mouse cerebellum tissue lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab92988 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF Use a concentration of 5 µg/ml. WB 1/250. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). Target
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Function
May be involved in neural development. Involved in regulation of osteoblast function and bone formation. Involved in matrix vesicle release by osteoblasts; this function seems to involve maintenance of the actin cytoskeleton. May be involved in cellular trafficking of SERT and thereby in regulation of serotonin uptake. -
Tissue specificity
Neurons and glia; cerebellar Bergmann glia, in glia within white matter tracts of the cerebellum and cerebrum, and in embryonic dorsal root ganglia. -
Sequence similarities
Belongs to the myelin proteolipid protein family. -
Cellular localization
Cell membrane. Colocalizes with SERT at the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 2824 Human
- Entrez Gene: 14758 Mouse
- Entrez Gene: 100173640 Orangutan
- Entrez Gene: 192179 Rat
- Omim: 300051 Human
- SwissProt: Q13491 Human
- SwissProt: P35803 Mouse
- SwissProt: Q5R603 Orangutan
see all -
Alternative names
- glycoprotein M6B antibody
- GPM6B antibody
- GPM6B_HUMAN antibody
see all
Images
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Western blot - Anti-GPM6B antibody (ab92988)Anti-GPM6B antibody (ab92988) at 1/250 dilution + Cerebellum Mouse Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 20 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-GPM6B antibody (ab92988)ICC/IF image of ab92988 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab92988 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (1)
ab92988 has been referenced in 1 publication.
- Choi KM et al. Distribution of the Immunoreactivity for Glycoprotein M6B in the Neurogenic Niche and Reactive Glia in the Injury Penumbra Following Traumatic Brain Injury in Mice. Exp Neurobiol 22:277-82 (2013). IHC . PubMed: 24465143
Images
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Western blot - Anti-GPM6B antibody (ab92988)Anti-GPM6B antibody (ab92988) at 1/250 dilution + Cerebellum Mouse Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 20 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-GPM6B antibody (ab92988)
ICC/IF image of ab92988 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab92988 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
