Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-GPD2 antibody [EPR14259] (ab188585)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14259] to GPD2
Suitable for: WB, Flow Cyt, IP
Reacts with: Human

Product name

Anti-GPD2 antibody [EPR14259]
See all GPD2 primary antibodies
Description

Rabbit monoclonal [EPR14259] to GPD2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HeLa cells; HeLa, MCF-7, NIH:OVCAR-3 and U87-MG cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14259
Isotype

IgG
Research areas

Western blot - Anti-GPD2 antibody [EPR14259] (ab188585)

All lanes : Anti-GPD2 antibody [EPR14259] (ab188585) at 1/10000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : NIH:OVCAR-3 cell lysate
Lane 4 : U87-MG cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 81 kDa
Observed band size: 74 kDa
why is the actual band size different from the predicted?
Flow Cytometry - Anti-GPD2 antibody [EPR14259] (ab188585)

Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling GPD2 with ab188585 at 1/310 dilution (red) compared to a rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-GPD2 antibody [EPR14259] (ab188585)

Western blot aalysis of GPD2 in HeLa cell lysate immunoprecipitated using ab188585 at 1/50 dilution.
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.
Anti-GPD2 antibody [EPR14259] (ab188585)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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