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Anti-GPD2 antibody [EPR14259] (ab188585)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14259] to GPD2
  • Suitable for: WB, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-GPD2 antibody [EPR14259]
    See all GPD2 primary antibodies

  • Description

    Rabbit monoclonal [EPR14259] to GPD2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa cells; HeLa, MCF-7, NIH:OVCAR-3 and U87-MG cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-GPD2 antibody [EPR14259] (ab188585)
    Western blot - Anti-GPD2 antibody [EPR14259] (ab188585)
    All lanes : Anti-GPD2 antibody [EPR14259] (ab188585) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF-7 cell lysate
    Lane 3 : NIH:OVCAR-3 cell lysate
    Lane 4 : U87-MG cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 81 kDa
    Observed band size: 74 kDa
    why is the actual band size different from the predicted?

  • Flow Cytometry - Anti-GPD2 antibody [EPR14259] (ab188585)
    Flow Cytometry - Anti-GPD2 antibody [EPR14259] (ab188585)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling GPD2 with ab188585 at 1/310 dilution (red) compared to a rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-GPD2 antibody [EPR14259] (ab188585)
    Immunoprecipitation - Anti-GPD2 antibody [EPR14259] (ab188585)

    Western blot aalysis of GPD2 in HeLa cell lysate immunoprecipitated using ab188585 at 1/50 dilution.

    Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

  • Anti-GPD2 antibody [EPR14259] (ab188585)
    Anti-GPD2 antibody [EPR14259] (ab188585)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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