Anti-GNAT2 antibody (ab97501)
Key features and details
- Rabbit polyclonal to GNAT2
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-GNAT2 antibody
See all GNAT2 primary antibodies -
Description
Rabbit polyclonal to GNAT2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in both Rat and Mouse Retina tissue lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97501 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseApplication Abreviews Notes WB (1) Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 40 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 40 kDa).Target
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Function
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase. -
Tissue specificity
Retinal rod outer segment. -
Involvement in disease
Defects in GNAT2 are the cause of achromatopsia type 4 (ACHM4) [MIM:139340]. Achromatopsia is an autosomal recessively inherited visual disorder that is present from birth and that features the absence of color discrimination. -
Sequence similarities
Belongs to the G-alpha family. G(i/o/t/z) subfamily. - Information by UniProt
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Database links
- Entrez Gene: 14686 Mouse
- Entrez Gene: 365901 Rat
- SwissProt: P50149 Mouse
- Unigene: 439652 Mouse
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Alternative names
- ACHM4 antibody
- Cone type transducin alpha subunit antibody
- GNAT 2 antibody
see all
Images
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Western blot - Anti-GNAT2 antibody (ab97501)All lanes : Anti-GNAT2 antibody (ab97501) at 1 µg/ml
Lane 1 : Mouse Retina Tissue Lysate
Lane 2 : Rat Retina Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Datasheets and documents
References (3)
ab97501 has been referenced in 3 publications.
- Maggi EC et al. Retinoblastoma-binding protein 2 (RBP2) is frequently expressed in neuroendocrine tumors and promotes the neoplastic phenotype. Oncogenesis 5:e257 (2016). PubMed: 27548814
- Datta P et al. Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome. Proc Natl Acad Sci U S A 112:E4400-9 (2015). WB ; Mouse . PubMed: 26216965
- Sato K et al. M-opsin protein degradation is inhibited by MG-132 in Rpe65(-/-) retinal explant culture. Mol Vis 18:1516-25 (2012). WB ; Mouse . PubMed: 22736942
Images
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Western blot - Anti-GNAT2 antibody (ab97501)All lanes : Anti-GNAT2 antibody (ab97501) at 1 µg/ml
Lane 1 : Mouse Retina Tissue Lysate
Lane 2 : Rat Retina Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
