Anti-GNAQ antibody [EPR17149] - BSA and Azide free (ab251282)
![Anti-GNAQ antibody [EPR17149] - BSA and Azide free (ab251282)](https://www.abcam.com/ps/products/251/ab251282/Images/ab251282-403496-anti-gnaq-antibody-epr17149-bsa-and-azide-free-western-blot.jpg "Anti-GNAQ antibody [EPR17149] - BSA and Azide free (ab251282)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17149] to GNAQ - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-GNAQ antibody [EPR17149] - BSA and Azide free
See all GNAQ primary antibodies -
Description
Rabbit monoclonal [EPR17149] to GNAQ - BSA and Azide free -
Host species
Rabbit -
Specificity
The immunogen used for this product shares 77% homology with GNA11. Cross-reactivity with this protein has not been confirmed experimentally.
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Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251282 is the carrier-free version of ab199533. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251282 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR17149 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GNAQ antibody [EPR17149] - BSA and Azide free (ab251282)All lanes : Anti-GNAQ antibody [EPR17149] (ab199533) at 1/1000 dilution
Lane 1 : HEK293 (Human embryonic kidney) whole cell lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 3 minutesThis data was developed using ab199533, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-GNAQ antibody [EPR17149] - BSA and Azide free (ab251282)](https://www.abcam.com/ps/products/251/ab251282/Images/ab251282-399127-anti-gnaq-antibody-epr17149-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-GNAQ antibody [EPR17149] - BSA and Azide free (ab251282)All lanes : Anti-GNAQ antibody [EPR17149] (ab199533) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat brain lysate
Lane 5 : Rat kidney lysate
Lane 6 : C6 (Rat glial tumor cells) whole cell lysate
Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 8 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 3 minutesThis data was developed using ab199533, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Anti-GNAQ antibody [EPR17149] - BSA and Azide free (ab251282)](https://www.abcam.com/ps/products/251/ab251282/Images/ab251282-3-benefits-of-recombinant-antibodies.png)
Anti-GNAQ antibody [EPR17149] - BSA and Azide free (ab251282)
