Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GNAI2 antibody [EPR9469] (ab157204)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9469] to GNAI2
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-GNAI2 antibody [EPR9469]
    See all GNAI2 primary antibodies

  • Description

    Rabbit monoclonal [EPR9469] to GNAI2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human GNAI2. The exact sequence is proprietary.
    Database link: P04899

  • Positive control

    • Recombinant Human GNAI2 (ab134531) can be used as a positive control in WB. U937 and U-87 MG cell lysates; fetal brain lysate; kidney and pancreas tissues; U937 cells.

  • General notes

     

     

     This product was previously labelled as G protein alpha Inhibitor 2

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR9469

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-GNAI2 antibody [EPR9469] (ab157204)
    Western blot - Anti-GNAI2 antibody [EPR9469] (ab157204)
    All lanes : Anti-GNAI2 antibody [EPR9469] (ab157204) at 1/10000 dilution

    Lane 1 : fetal brain lysate
    Lane 2 : U937 cell lysate
    Lane 3 : U-87 MG cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 40 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI2 antibody [EPR9469] (ab157204)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI2 antibody [EPR9469] (ab157204)

    ab157204 showing +ve staining in Human normal liver.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI2 antibody [EPR9469] (ab157204)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI2 antibody [EPR9469] (ab157204)

    ab157204 showing +ve staining in Human normal brain.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI2 antibody [EPR9469] (ab157204)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI2 antibody [EPR9469] (ab157204)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling GNAI2 using ab157204 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI2 antibody [EPR9469] (ab157204)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI2 antibody [EPR9469] (ab157204)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, labeling GNAI2 using ab157204 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-GNAI2 antibody [EPR9469] (ab157204)
    Flow Cytometry - Anti-GNAI2 antibody [EPR9469] (ab157204)

    Flow cytometry analysis of permeabilized U937 cells labeling GNAI2 (red), using ab157204 at a 1/10 dilution, and negative control cells probed with a Rabbit IgG (green)

  • Anti-GNAI2 antibody [EPR9469] (ab157204)
    Anti-GNAI2 antibody [EPR9469] (ab157204)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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