Anti-GMFB antibody (ab224322)
Key features and details
- Rabbit polyclonal to GMFB
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-GMFB antibody
See all GMFB primary antibodies -
Description
Rabbit polyclonal to GMFB -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Orangutan
-
Immunogen
Recombinant fragment corresponding to Human GMFB aa 1 to the C-terminus.
Database link: P60983 -
Positive control
- ICC/IF: U-251 MG cells. WB: NIH/3T3, A431 and NBT-II cell lysates; Human and mouse cerebral cortex tissue lysates. IHC-P: Mouse somatosensory cortex, olfactory bulb, lateral septum, red nucleus and cerebellum tissues; Human cerebral cortex and hippocampus tissues.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (ab224322)
Paraffin-embedded mouse cerebellum tissue stained for GMFB using ab224322 at 1/200 dilution in immunohistochemical analysis using a fluorescent secondary antibody.
-
Immunocytochemistry/ Immunofluorescence - Anti-GMFB antibody (ab224322)PFA-fixed/Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for GMFB (green) using ab224322 at 4 µg/ml in ICC/IF.
-
Western blot - Anti-GMFB antibody (ab224322)Anti-GMFB antibody (ab224322) at 1/100 dilution + A431 (human epidermoid carcinoma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 17 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (ab224322)Paraffin-embedded human hippocampus tissue stained for GMFB using ab224322 at 1/200 dilution in immunohistochemical analysis.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (ab224322)Paraffin-embedded mouse red nucleus tissue stained for GMFB using ab224322 at 1/200 dilution in immunohistochemical analysis using a fluorescent secondary antibody.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (ab224322)Paraffin-embedded mouse lateral septum tissue stained for GMFB using ab224322 at 1/200 dilution in immunohistochemical analysis using a fluorescent secondary antibody.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (ab224322)Paraffin-embedded mouse olfactory bulb tissue stained for GMFB using ab224322 at 1/200 dilution in immunohistochemical analysis using a fluorescent secondary antibody.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (ab224322)Paraffin-embedded human cerebral cortex tissue stained for GMFB using ab224322 at 1/200 dilution in immunohistochemical analysis.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (ab224322)Paraffin-embedded mouse somatosensory cortex tissue stained for GMFB using ab224322 at 1/200 dilution in immunohistochemical analysis using a fluorescent secondary antibody.
-
Western blot - Anti-GMFB antibody (ab224322)Anti-GMFB antibody (ab224322) at 1/100 dilution + Human cerebral cortex tissue lysate
Developed using the ECL technique.
Predicted band size: 17 kDa
-
Western blot - Anti-GMFB antibody (ab224322)Anti-GMFB antibody (ab224322) at 1/100 dilution + Mouse cerebral cortex tissue lysate
Developed using the ECL technique.
Predicted band size: 17 kDa
-
Western blot - Anti-GMFB antibody (ab224322)All lanes : Anti-GMFB antibody (ab224322) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate
Lane 2 : NBT-II cell lysate
Developed using the ECL technique.
Predicted band size: 17 kDa
