Anti-Glutathione Peroxidase 4 antibody (ab41787)
Key features and details
- Rabbit polyclonal to Glutathione Peroxidase 4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Glutathione Peroxidase 4 antibody
See all Glutathione Peroxidase 4 primary antibodies -
Description
Rabbit polyclonal to Glutathione Peroxidase 4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Glutathione Peroxidase 4 aa 150 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab41786) -
Positive control
- ab41787 gave a positive signal in the following human tissue lysates: Brain, Testis, Heart, Skeletal Muscle, Kidney, Colon, Liver, Small Intestine (data not shown for Liver and Small Intestine).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab41787 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications a wide range of other speciesApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).Blocking: we recommend using 3% milk block for 1 hour at room temperature. This step may need to be optimized for your experiments.
Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).Blocking: we recommend using 3% milk block for 1 hour at room temperature. This step may need to be optimized for your experiments.
Target
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Function
Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage. -
Tissue specificity
Present primarily in testis. -
Sequence similarities
Belongs to the glutathione peroxidase family. -
Cellular localization
Mitochondrion. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 2879 Human
- Omim: 138322 Human
- SwissProt: P36969 Human
- Unigene: 433951 Human
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Alternative names
- Glutathione peroxidase 4 antibody
- GPX 4 antibody
- GPX-4 antibody
see all
Images
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Western blot - Anti-Glutathione Peroxidase 4 antibody (ab41787)All lanes : Anti-Glutathione Peroxidase 4 antibody (ab41787) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human testis tissue lysate - total protein (ab30257)
Lane 3 : Human heart tissue lysate - total protein (ab29431)
Lane 4 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 5 : Human kidney tissue lysate - total protein (ab30203)
Lane 6 : Human colon tissue lysate - total protein (ab30051)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Additional bands at: 27 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Protocols
Datasheets and documents
References (12)
ab41787 has been referenced in 12 publications.
- Weigand I et al. Active steroid hormone synthesis renders adrenocortical cells highly susceptible to type II ferroptosis induction. Cell Death Dis 11:192 (2020). PubMed: 32184394
- Li M et al. Metal ion-responsive nanocarrier derived from phosphonated calix[4]arenes for delivering dauricine specifically to sites of brain injury in a mouse model of intracerebral hemorrhage. J Nanobiotechnology 18:61 (2020). PubMed: 32306970
- Garcia-Bermudez J et al. Squalene accumulation in cholesterol auxotrophic lymphomas prevents oxidative cell death. Nature 567:118-122 (2019). PubMed: 30760928
- Zou Y et al. A GPX4-dependent cancer cell state underlies the clear-cell morphology and confers sensitivity to ferroptosis. Nat Commun 10:1617 (2019). PubMed: 30962421
- Lang X et al. Radiotherapy and Immunotherapy Promote Tumoral Lipid Oxidation and Ferroptosis via Synergistic Repression of SLC7A11. Cancer Discov 9:1673-1685 (2019). PubMed: 31554642
Images
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Western blot - Anti-Glutathione Peroxidase 4 antibody (ab41787)All lanes : Anti-Glutathione Peroxidase 4 antibody (ab41787) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human testis tissue lysate - total protein (ab30257)
Lane 3 : Human heart tissue lysate - total protein (ab29431)
Lane 4 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 5 : Human kidney tissue lysate - total protein (ab30203)
Lane 6 : Human colon tissue lysate - total protein (ab30051)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Additional bands at: 27 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
