Anti-Glutathione Peroxidase 4 antibody (ab40993)
Key features and details
- Rabbit polyclonal to Glutathione Peroxidase 4
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Glutathione Peroxidase 4 antibody
See all Glutathione Peroxidase 4 primary antibodies -
Description
Rabbit polyclonal to Glutathione Peroxidase 4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
-
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Glutathione Peroxidase 4.
Read Abcam's proprietary immunogen policy (Peptide available as ab41785.) -
Positive control
- ab40993 gave a positive signal in the following human tissue lysates: Brain, Testis, Liver, Heart, Skeletal Muscle, Kidney, Colon, Small Inestine (data not shown).
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab40993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanAll applications a wide range of other speciesApplication Abreviews Notes ICC/IF Use a concentration of 1 µg/ml.WB Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).Notes ICC/IF
Use a concentration of 1 µg/ml.WB
Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).Target
-
Function
Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage. -
Tissue specificity
Present primarily in testis. -
Sequence similarities
Belongs to the glutathione peroxidase family. -
Cellular localization
Mitochondrion. Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 2879 Human
- Omim: 138322 Human
- SwissProt: P36969 Human
- Unigene: 433951 Human
-
Alternative names
- Glutathione peroxidase 4 antibody
- GPX 4 antibody
- GPX-4 antibody
see all
Images
-
Western blot - Anti-Glutathione Peroxidase 4 antibody (ab40993)All lanes : Anti-Glutathione Peroxidase 4 antibody (ab40993) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human testis tissue lysate - total protein (ab30257)
Lane 3 : Human liver tissue lysate - total protein (ab29889)
Lane 4 : Human heart tissue lysate - total protein (ab29431)
Lane 5 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 6 : Human kidney tissue lysate - total protein (ab30203)
Lane 7 : Human colon tissue lysate - total protein (ab30051)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 22 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Glutathione Peroxidase 4 antibody (ab40993)ICC/IF image of ab40993 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab40993, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Protocols
References (3)
ab40993 has been referenced in 3 publications.
- O'Brien KM et al. The loss of hemoglobin and myoglobin does not minimize oxidative stress in Antarctic icefishes. J Exp Biol 221:N/A (2018). PubMed: 29361578
- Hauser DN et al. Dopamine quinone modifies and decreases the abundance of the mitochondrial selenoprotein glutathione peroxidase 4. Free Radic Biol Med 65:419-27 (2013). PubMed: 23816523
- Li W et al. Repression of endometrial tumor growth by targeting SREBP1 and lipogenesis. Cell Cycle 11:2348-58 (2012). PubMed: 22672904
Images
-
Western blot - Anti-Glutathione Peroxidase 4 antibody (ab40993)All lanes : Anti-Glutathione Peroxidase 4 antibody (ab40993) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human testis tissue lysate - total protein (ab30257)
Lane 3 : Human liver tissue lysate - total protein (ab29889)
Lane 4 : Human heart tissue lysate - total protein (ab29431)
Lane 5 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 6 : Human kidney tissue lysate - total protein (ab30203)
Lane 7 : Human colon tissue lysate - total protein (ab30051)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 22 kDa
-
Immunocytochemistry/ Immunofluorescence - Anti-Glutathione Peroxidase 4 antibody (ab40993)ICC/IF image of ab40993 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab40993, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
