Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6635] to Lipoamide Dehydrogenase
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Lipoamide Dehydrogenase antibody [EPR6635]
    See all Lipoamide Dehydrogenase primary antibodies

  • Description

    Rabbit monoclonal [EPR6635] to Lipoamide Dehydrogenase

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to residues in Human Lipoamide Dehydrogenase (UniProt ID: P09622).

  • Positive control

    • WB: Jurkat, HeLa, 293T, MCF7, and Caco-2 cell lysates. IHC-P: Human kidney and Human colon tissues

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)
    Western blot - Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)
    All lanes : Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : DLD knockout HeLa cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 54 kDa
    Observed band size: 56 kDa
    why is the actual band size different from the predicted?



    Lanes 1-3: Merged signal (red and green). Green - ab133551 observed at 56 kDa. Red - loading control ab8245 observed at 36 kDa.

    ab133551 Anti-Lipoamide Dehydrogenase antibody [EPR6635] was shown to specifically react with Lipoamide Dehydrogenase in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265535 (knockout cell lysate ab257922) was used. Wild-type and Lipoamide Dehydrogenase knockout samples were subjected to SDS-PAGE. ab133551 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)

    Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Lipoamide Dehydrogenase with ab133551 antibody at a dilution of 1/100.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)
    Western blot - Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)
    All lanes : Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : MCF7 cell lysate
    Lane 5 : Caco-2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 54 kDa
    Observed band size: 56 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)

    Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Lipoamide Dehydrogenase with ab133551 antibody at a dilution of 1/100.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)
    OI-RD Scanning - Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)
    Anti-Lipoamide Dehydrogenase antibody [EPR6635] (ab133551)

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