Anti-Glucose Oxidase antibody (ab181638)
Key features and details
- Goat polyclonal to Glucose Oxidase
- Suitable for: IHC-P, WB
- Isotype: IgG
Overview
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Product name
Anti-Glucose Oxidase antibody
See all Glucose Oxidase primary antibodies -
Description
Goat polyclonal to Glucose Oxidase -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Other species -
Immunogen
Full length native protein (purified) corresponding to Glucose Oxidase aa 23-605. (Aspergillus niger).
Sequence:SNGIEASLLTDPKDVSGRTVDYIIAGGGLTGLTTAARLTENPNISVLVIE SGSYESDRGPIIEDLNAYGDIFGSSVDHAYETVELATNNQTALIRSGNGL GGSTLVNGGTWTRPHKAQVDSWETVFGNEGWNWDNVAAYSLQAERARAPN AKQIAAGHYFNASCHGVNGTVHAGPRDTGDDYSPIVKALMSAVEDRGVPT KKDFGCGDPHGVSMFPNTLHEDQVRSDAAREWLLPNYQRPNLQVLTGQYV GKVLLSQNGTTPRAVGVEFGTHKGNTHNVYAKHEVLLAAGSAVSPTILEY SGIGMKSILEPLGIDTVVDLPVGLNLQDQTTATVRSRITSAGAGQGQAAW FATFNETFGDYSEKAHELLNTKLEQWAEEAVARGGFHNTTALLIQYENYR DWIVNHNVAYSELFLDTAGVASFDVWDLLPFTRGYVHILDKDPYLHHFAY DPQYFLNELDLLGQAAATQLARNISNSGAMQTYFAGETIPGDNLAYDADL SAWTEYIPYHFRPNYHGVGTCSMMPKEMGGVVDNAARVYGVQGLRVIDGS IPPTQMSSHVMTVFYAMALKISDAILEDYASMQ
Database link: P13006 -
Positive control
- Purified Glucose Oxidase protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.27% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
ab181638 is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Glucose Oxidase antibody (ab181638)All lanes : Anti-Glucose Oxidase antibody (ab181638) at 1/5000 dilution
Lanes 1 & 3 : Glucose Oxidase protein at 1 µg
Lanes 2 & 4 : Glucose Oxidase protein at 0.25 µg
Secondary
All lanes : Dylight 649 conjugated Donkey anti Goat at 1/10000 dilution
Predicted band size: 66 kDaLane 1 + 2 under reducing and Lane 3 +4 under non-reducing conditions.
Reduced samples of purified Glucose Oxidase contained 4% BME and were boiled for 5 minutes.
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Western blot - Anti-Glucose Oxidase antibody (ab181638)Anti-Glucose Oxidase antibody (ab181638) at 1/1000 dilution + Glucose Oxidase protein at 0.05 µg
Secondary
Peroxidase anti-goat secondary antibody at 1/40000 dilution
Predicted band size: 66 kDa
Observed band size: 65-70 kDa why is the actual band size different from the predicted?