Anti-Glucagon Receptor antibody (ab109279)
Key features and details
- Rabbit polyclonal to Glucagon Receptor
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Glucagon Receptor antibody
See all Glucagon Receptor primary antibodies -
Description
Rabbit polyclonal to Glucagon Receptor -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Glucagon Receptor aa 300-400 conjugated to keyhole limpet haemocyanin.
(Peptide available asab129210) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: Caco2; HepG2; SW480.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications Macaque monkeyGorillaApplication Abreviews Notes WB 1/250. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).Notes WB
1/250. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).Target
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Function
This is a receptor for glucagon which plays a central role in regulating the level of blood glucose by controlling the rate of hepatic glucose production and insulin secretion. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system. -
Sequence similarities
Belongs to the G-protein coupled receptor 2 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2642 Human
- Omim: 138033 Human
- SwissProt: P47871 Human
- Unigene: 208 Human
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Alternative names
- Gcgr antibody
- GGR antibody
- GL R antibody
see all
Images
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Western blot - Anti-Glucagon Receptor antibody (ab109279)All lanes : Anti-Glucagon Receptor antibody (ab109279) at 1/250 dilution (Milk Blocking - 3%)
Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Additional bands at: 25 kDa, 51 kDa (possible cleavage fragment). We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
The band observed at 51 kDa could potentially be a cleaved form of Glucagon Receptor due to the presence of a 25 amino acid signal peptide.
Datasheets and documents
References (0)
ab109279 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Glucagon Receptor antibody (ab109279)All lanes : Anti-Glucagon Receptor antibody (ab109279) at 1/250 dilution (Milk Blocking - 3%)
Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Additional bands at: 25 kDa, 51 kDa (possible cleavage fragment). We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
The band observed at 51 kDa could potentially be a cleaved form of Glucagon Receptor due to the presence of a 25 amino acid signal peptide.
