Anti-GLS2 antibody (ab150474)
Key features and details
- Rabbit polyclonal to GLS2
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-GLS2 antibody
See all GLS2 primary antibodies -
Description
Rabbit polyclonal to GLS2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Rabbit, Cow, Dog, Human, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide corresponding to Human GLS2 aa 550 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: Q9UI32 -
Positive control
- This antibody gave a positive signal in Mouse Liver tissue lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab150474 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa). Target
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Function
Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation. -
Tissue specificity
Highly expressed in liver. Expressed in brain and pancreas. Not observed in heart, placenta, lung, skeletal muscle and kidney. Expression is significantly reduced in hepatocellular carcinomas. -
Sequence similarities
Belongs to the glutaminase family.
Contains 2 ANK repeats. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 27165 Human
- Entrez Gene: 216456 Mouse
- Entrez Gene: 192268 Rat
- Omim: 606365 Human
- SwissProt: Q9UI32 Human
- SwissProt: Q571F8 Mouse
- SwissProt: P28492 Rat
- Unigene: 212606 Human
see all -
Alternative names
- breast cell glutaminase antibody
- GA antibody
- GLS antibody
see all
Images
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Western blot - Anti-GLS2 antibody (ab150474)Anti-GLS2 antibody (ab150474) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 66 kDa
Observed band size: 66 kDa
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab150474 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (2)
ab150474 has been referenced in 2 publications.
- Huang X et al. The HGF-MET axis coordinates liver cancer metabolism and autophagy for chemotherapeutic resistance. Autophagy 15:1258-1279 (2019). PubMed: 30786811
- Lukey MJ et al. Liver-Type Glutaminase GLS2 Is a Druggable Metabolic Node in Luminal-Subtype Breast Cancer. Cell Rep 29:76-88.e7 (2019). PubMed: 31577957
Images
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Western blot - Anti-GLS2 antibody (ab150474)Anti-GLS2 antibody (ab150474) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 66 kDa
Observed band size: 66 kDa
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab150474 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
