Anti-GIGYF1 antibody (ab177509)
Key features and details
- Rabbit polyclonal to GIGYF1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GIGYF1 antibody
See all GIGYF1 primary antibodies -
Description
Rabbit polyclonal to GIGYF1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human GIGYF1 aa 350-400. The exact sequence is proprietary. (NP_072096.2)
Sequence:E AGGKELTPLP PQEEKSSSPS PLPTLGPLWG TNGDGDETAE KEPPAAEDDI
Database link: O75420 -
Positive control
- HeLa, 293T and Jurkat whole cell lysate (ab7899).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 - 8. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab177509 was affinity purified using an epitope specific to GIGYF1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GIGYF1 antibody (ab177509)All lanes : Anti-GIGYF1 antibody (ab177509) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 115 kDa
Exposure time: 10 seconds
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Immunoprecipitation - Anti-GIGYF1 antibody (ab177509)Detection of GIGYF1 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab177509 at 6 µg/mg lysate for IP (Lane 1). For WB detection, ab177509 was used at 0.4 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
