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Anti-GFM1 antibody [EPR12263-33] - BSA and Azide free (ab249767)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12263-33] to GFM1 - BSA and Azide free
  • Suitable for: ICC/IF, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-GFM1 antibody [EPR12263-33] - BSA and Azide free
    See all GFM1 primary antibodies

  • Description

    Rabbit monoclonal [EPR12263-33] to GFM1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IPmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Recombinant fragment within Human GFM1. The exact sequence is proprietary.
    Database link: Q96RP9

  • General notes

    ab249767 is the carrier-free version of ab173529.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-GFM1 antibody [EPR12263-33] - BSA and Azide free (ab249767)
    Western blot - Anti-GFM1 antibody [EPR12263-33] - BSA and Azide free (ab249767)
    All lanes : Anti-GFM1 antibody [EPR12263-33] (ab173529) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : NCI-H460 cell lysate
    Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
    Lane 4 : 293T (Human embryonic kidney epithelial cell) cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 83 kDa



    This data was developed using ab173529, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-GFM1 antibody [EPR12263-33] - BSA and Azide free (ab249767)
    Immunocytochemistry - Anti-GFM1 antibody [EPR12263-33] - BSA and Azide free (ab249767)
    This data was developed using ab173529, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of HeLa cells labeling GFM1 with ab173529 at a 1/50 dilution.
  • Immunoprecipitation - Anti-GFM1 antibody [EPR12263-33] - BSA and Azide free (ab249767)
    Immunoprecipitation - Anti-GFM1 antibody [EPR12263-33] - BSA and Azide free (ab249767)
    This data was developed using ab173529, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from HeLa cell lysate: ab173529 at a 1/10 dilution was used for immunoprecipitate GFM1.
  • Anti-GFM1 antibody [EPR12263-33] - BSA and Azide free (ab249767)
    Anti-GFM1 antibody [EPR12263-33] - BSA and Azide free (ab249767)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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