Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Gemin 5 antibody [EPR17841] - BSA and Azide free (ab251353)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17841] to Gemin 5 - BSA and Azide free
  • Suitable for: Flow Cyt, ICC, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Gemin 5 antibody [EPR17841] - BSA and Azide free
    See all Gemin 5 primary antibodies

  • Description

    Rabbit monoclonal [EPR17841] to Gemin 5 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC, WB, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251353 is the carrier-free version of ab201691. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251353 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Gemin 5 antibody [EPR17841] - BSA and Azide free (ab251353)
    Western blot - Anti-Gemin 5 antibody [EPR17841] - BSA and Azide free (ab251353)
    All lanes : Anti-Gemin 5 antibody [EPR17841] (ab201691) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
    Lane 2 : K562 (Human chronic myelogenous leukemia cells from bone marrow) cell lysate
    Lane 3 : HEK-293 (Human epithelial cells from embryonic kidney) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 168 kDa
    Observed band size: 168 kDa


    Exposure time: 7 seconds


    This data was developed using ab201691, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-Gemin 5 antibody [EPR17841] - BSA and Azide free (ab251353)
    Immunocytochemistry - Anti-Gemin 5 antibody [EPR17841] - BSA and Azide free (ab251353)
    This data was developed using ab201691, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Gemin 5 with ab201691 at 1/600 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows;
    -ve control 1: ab201691 at 1/600 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.
  • Immunocytochemistry - Anti-Gemin 5 antibody [EPR17841] - BSA and Azide free (ab251353)
    Immunocytochemistry - Anti-Gemin 5 antibody [EPR17841] - BSA and Azide free (ab251353)
    This data was developed using ab201691, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling Gemin 5 with ab201691 at 1/600 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on Jurkat cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows;
    -ve control 1: ab201691 at 1/600 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.
  • Flow Cytometry - Anti-Gemin 5 antibody [EPR17841] - BSA and Azide free (ab251353)
    Flow Cytometry - Anti-Gemin 5 antibody [EPR17841] - BSA and Azide free (ab251353)
    This data was developed using ab201691, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed HEK-293 (Human epithelial cells from embryonic kidney) cells labeling Gemin 5 with ab201691 at 1/250 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
  • Immunoprecipitation - Anti-Gemin 5 antibody [EPR17841] - BSA and Azide free (ab251353)
    Immunoprecipitation - Anti-Gemin 5 antibody [EPR17841] - BSA and Azide free (ab251353)

    This data was developed using ab201691, the same antibody clone in a different buffer formulation.

    Gemin 5 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab201691 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab201691 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500 dilution.

    Lane 1: HeLa whole cell lysate 10 µg (Input). Lane 2: ab201691 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab201691 in HeLa whole cell lysate.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 seconds.

  • Anti-Gemin 5 antibody [EPR17841] - BSA and Azide free (ab251353)
    Anti-Gemin 5 antibody [EPR17841] - BSA and Azide free (ab251353)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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