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Anti-GCH1 antibody [EPR24155-33] - BSA and Azide free (ab280368)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24155-33] to GCH1 - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P
  • Reacts with: Rat

Overview

  • Product name

    Anti-GCH1 antibody [EPR24155-33] - BSA and Azide free
    See all GCH1 primary antibodies

  • Description

    Rabbit monoclonal [EPR24155-33] to GCH1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IHC-Pmore details
    Unsuitable for: ICC,IHC-Fr,IP or WB

  • Species reactivity

    Reacts with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Rat brain and stomach tissues. Flow Cyt: Rat primary neuron and glia cell.

  • General notes

    ab280368 is the carrier-free version of ab259882. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280368 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR24155-33] - BSA and Azide free (ab280368)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR24155-33] - BSA and Azide free (ab280368)

    This data was developed using ab259882, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat brain tissue labelling GCH1 with ab259882 at 1/500 (1.278 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on ventral tegmental area of rat brain. The section was incubated with ab259882 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR24155-33] - BSA and Azide free (ab280368)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR24155-33] - BSA and Azide free (ab280368)

    This data was developed using ab259882, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat brain tissue labelling GCH1 with ab259882 at 1/500 (1.278 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on ventral tegmental area of rat brain. The section was incubated with ab259882 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR24155-33] - BSA and Azide free (ab280368)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR24155-33] - BSA and Azide free (ab280368)

    This data was developed using ab259882, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labelling GCH1 with ab259882 at 1/500 (1.278 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat stomach. The section was incubated with ab259882 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Flow Cytometry - Anti-GCH1 antibody [EPR24155-33] - BSA and Azide free (ab280368)
    Flow Cytometry - Anti-GCH1 antibody [EPR24155-33] - BSA and Azide free (ab280368)

    This data was developed using ab259882, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Rat primary neuron and glia cells cells labelling GCH1 with ab259882 at 1/500 dilution (0.1ug). Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Anti-GCH1 antibody [EPR24155-33] - BSA and Azide free (ab280368)
    Anti-GCH1 antibody [EPR24155-33] - BSA and Azide free (ab280368)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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